. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ERYTHROCYTE SHAPE TRANSFORMATION 397. Figure 1. Light micrographs of Noelia ponderosa erythrocytes before and after shape transformation, (a) Normal erythrocytes (N-cells), flattened and slightly ellipsoidal; (b) shape-transformed erythrocytes (X-cells), appearing refractile, lumpy, and spheroidal at this level of resolution: (c. d) examples of shape-transformed erythrocytes at different stages during recovery: (e) cells of essentially normal shape post-recovery. Video- enhanced phase contrast microscopy; bar = 10 fxm. 10 mg
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ERYTHROCYTE SHAPE TRANSFORMATION 397. Figure 1. Light micrographs of Noelia ponderosa erythrocytes before and after shape transformation, (a) Normal erythrocytes (N-cells), flattened and slightly ellipsoidal; (b) shape-transformed erythrocytes (X-cells), appearing refractile, lumpy, and spheroidal at this level of resolution: (c. d) examples of shape-transformed erythrocytes at different stages during recovery: (e) cells of essentially normal shape post-recovery. Video- enhanced phase contrast microscopy; bar = 10 fxm. 10 mg/ml tibrinogen (Sigma F-8630). Thrombin (Sigma T-4648) was added to 1 U/ml to produce a firm clot in about 10 min; within this time window, 5- to 25-jul samples were spread inside plastic rings adhering to coverslips. Cytoskel- etons were produced by immersing coverslips bearing fi- brin-trapped cells in Triton lysis medium (see above) for 1 min, followed by fixation in PEM containing 8% formalde- hyde. In some cases, 4% formaldehyde was included in the lysis medium for additional stabilization. This was followed by PBS washes and incubation with a 50:50 mix of mono- clonal anti-a- and anti-/3-tubulin (Sigma T-9026, T-4026). using secondary FITC goat anti-mouse IgG F(ab'), (Sigma F-8521). Fibrin-trapped specimens were examined using epifluorescence and confocal fluorescence microscopy. Controls lacking primary antibody or phalloidin exhibited little background fluorescence. For scanning electron microscopy, cells were fixed for 1 h in 16% formaldehyde in M KH-,PO4, pH , at 21°-23°C (room temperature); dehydrated in ethanol to 70%; incubated on acid-cleaned polylysine-coated cov- erslips; dehydrated to 100% ethanol; and critical-point dried (Tousimis Samdri-780A). Coverslips were mounted on stubs with double-sided tape and sputter coated with Au/Pd (Tousimis Samsputter 2A), with silver paste added for conductivity. Hemolymph dialysis, filtration, ami chromatography Hemo
Size: 2878px × 868px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
