. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 554 KENXEY, BELAMARICH AND SHEPRO Oxalate inhibition of amebocyte aggregation is not suited to photometric ex- amination, for, even at low concentrations (2 ± I HIM), buffered oxalate causes expulsion of intact, free granules that produce turbidity and mask increases in transmission resulting from aggregation. As evaluated by microscopic observation, oxalate inhibition of amebocyte aggregation is intermediate between the inhibition produced by EDTA and citrate. When examined microscopically amebocytes in 17 ± 6 HIM EDTA appe
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 554 KENXEY, BELAMARICH AND SHEPRO Oxalate inhibition of amebocyte aggregation is not suited to photometric ex- amination, for, even at low concentrations (2 ± I HIM), buffered oxalate causes expulsion of intact, free granules that produce turbidity and mask increases in transmission resulting from aggregation. As evaluated by microscopic observation, oxalate inhibition of amebocyte aggregation is intermediate between the inhibition produced by EDTA and citrate. When examined microscopically amebocytes in 17 ± 6 HIM EDTA appear in- tact and show little evidence of the extensive alterations characteristic of aggrega- tion (Fig. 3 ). EDTA treated amebocytes remain ovoid and contain the character- istic granules (Fig. 3A), whereas, amebocytes in saline at 2 in in (Fig. 3B) 100- 90- Q 6. 0 4a. 105 120 TIME ( sec) FIGURE 4. Representative experiments showing the effect of calcium (4a) and magnesium (4b) on amebocytes in EDTA; (4a) control aggregation in saline (•), amebocytes in 17 + 6 HIM EDTA (O) : amebocytes in 17 + 6 imi EDTA added to: 8± 1 HIM calcium (A) ; 15 ± 2 mM calcium (•). 24 ± 2 HIM calcium (A), 32 ± 3 mM calcium (D). (4b) Control aggregation in saline (•); amebocytes in 17 ± 6 HIM EDTA (O). Amebocytes in 17 ±6 mM EDTA added to: 8±1 mil magnesium (A), 15 ± 2 HIM magnesium (•), 24 ± 2 mM magnesium ( A).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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