. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 92 T. Table I Si:e of algal and bacterial cells used for feeding experiments Type ESD (Mm) Phaeodactvlum incornuntm Duna/iel/a lerliolecla Tftraselmis sp. Cyanobacteria. ACMM326 Cvanobactena — small strain Natural bacteria — L: Length of long axis, ESD: equivalent spherical diameter. mixtures of FLC and living cells of the algae P. tricor- inititm. D. lerliolecla, and Tetraselmis sp. and two species of cultured cyanobacteria at various proportions for 5- 10 min. FLC
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 92 T. Table I Si:e of algal and bacterial cells used for feeding experiments Type ESD (Mm) Phaeodactvlum incornuntm Duna/iel/a lerliolecla Tftraselmis sp. Cyanobacteria. ACMM326 Cvanobactena — small strain Natural bacteria — L: Length of long axis, ESD: equivalent spherical diameter. mixtures of FLC and living cells of the algae P. tricor- inititm. D. lerliolecla, and Tetraselmis sp. and two species of cultured cyanobacteria at various proportions for 5- 10 min. FLC and living cells in the gut were counted immediately after experiments, because the autofluores- cence of living cells faded rapidly when stored. Feeding rate FLC uptake was determined for larvae at the late bipin- naria and early and late brachiolaria stages. Fifteen larvae were introduced into each experimental vial with 20 ml of filtered seawater. After about 2 h, 80-2000 jul of FLC suspension was added to each vial. As described below, larvae ingested FLC selectively over living cells of Tetra- selmis sp. Therefore, Tetraselmis sp. was no longer used in this series of experiments. The concentration range of FLC was X 101 cells ml ' for P. triconnawn. X 10' cells ml ' for D. lertiolecta, X 104 cells ml~' for cyanobacteria ACMM326, X 104 cells mr1 for unidentified cyanobacteria, and X 105 cells ml"1 for natural bacteria. As shown in Table I, the equivalent spherical diameter (ESD) ranged between 1 and about 5 ^m. The duration of incubation was chosen between 3 and 10 min., depending on the type and concentration of FLC. Samples obtained were preserved and processed as described in the measurements of gut filling rates. Results Toxicity of FLC No larvae died during 2-day incubations in either FLC or control treatment, although a few deformed larvae were observed. All larvae observed under an epifluorescence microscop
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
