. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . and inner part of the. thigh The skin is loosened by cutting around the lower portion ofthe trunk. It is then pulled by the hands toward the upper extremityof the animal and over the head to the ears, leaving the back exposed RABIES 571 and sterile throughout the entire length of the spine. The spine isthen divided transversely near each extremity by bone-cutting muscles are cut through about these areas so the spine may bemore easily reached. With a long wire probe swabbed wit
. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . and inner part of the. thigh The skin is loosened by cutting around the lower portion ofthe trunk. It is then pulled by the hands toward the upper extremityof the animal and over the head to the ears, leaving the back exposed RABIES 571 and sterile throughout the entire length of the spine. The spine isthen divided transversely near each extremity by bone-cutting muscles are cut through about these areas so the spine may bemore easily reached. With a long wire probe swabbed with cottonat one end the cord is pushed upward from its canal, freed from itsnerves and membranes. The spine is steadied by lion-jawed cord curls in a spiral as it emerges and rests on the sterile musclesof the neck. It is lifted with forceps, placed in a Petri dish and cutin two. A small piece is cut from one end and is dropped into a tubeof broth to test its purity. A ligature with one long end is placed abouteach piece, both of which are then hung in a drying bottle (Fig. 197).. Fig. -One corner of constant temperature room showing drying bottle containing fixedvirus cords being prepared for vaccine. Drying the Cord.—^The drying bottles are sterile aspiration bottleswith both openings plugged with cotton. A layer 1 inch high of sticksof caustic potash covers the bottom, and the pieces of cord are suspendedfrom the top cotton plug by their attached ligatures. The bottles arethen labeled and placed in the constant temperature room (Fig. 197)ot incubator, which is kept at a temperature of about 22° C. (70° F.).After twenty-four hours drying the cord is known as one-day cord;after two days, two-day cord, etc. Pieces of cord cut off at any time andput into glycerin will retain about the same strength for several procedure is followed in regions where there are few cases of rabies,and the daily killing of rabbits to keep up the vaccine would be a la
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