. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 384 M. M. X1JMOUT AND L. M. RIDDIFORD CO O> en o> cu o E c i o JC CO 0 I o 100 r » • A 120 c: o to o o> o CO o. 12 24 36 48 60 72 Hours after eclosion 84 96 FIGURE 3. Activity of the CA after adult eclosion. Each point represents observations on 51 ± 3 animals except that at 18 hours which represents 20 animals, (A) per cent of animals which have activated CA by time of decapitation at various times after eclosion; (B) number of eggs matured by these decapitated females by 96 hours ±; (C) number of mature eggs
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 384 M. M. X1JMOUT AND L. M. RIDDIFORD CO O> en o> cu o E c i o JC CO 0 I o 100 r » • A 120 c: o to o o> o CO o. 12 24 36 48 60 72 Hours after eclosion 84 96 FIGURE 3. Activity of the CA after adult eclosion. Each point represents observations on 51 ± 3 animals except that at 18 hours which represents 20 animals, (A) per cent of animals which have activated CA by time of decapitation at various times after eclosion; (B) number of eggs matured by these decapitated females by 96 hours ±; (C) number of mature eggs present in intact females at various times after eclosion ± necessary for ovarian development. Therefore, brainless female moths were used as assay subjects for JH. As soon as the brainless animal emerged, it received an injection of 5 TTX. Twelve hours later, 50 ^,1 of a JH solution was in- jected; then the animal was dissected at 96 hours. Controls received 50 /ul of pure mineral oil. Table III shows that individuals deprived of their brains throughout adult development responded as well to injected JH as did females decapitated after eclosion. Activity of the CA in adult females In order to study the activation of the CA after eclosion and the kinetics of JH release within the population, large numbers of animals were held for various periods of time after emergence and then decapitated. All were dissected on the fourth day after eclosion and the number of mature eggs counted. The results are shown in Figure 3. Curve A shows that the ability of females to form mature eggs increased when decapitation was performed progressively later in adult life. At the time of emergence, 19% of the population was competent to mature a few eggs (average of 9 eggs) without the continued presence of the CA. Therefore, only a small amount of hormone had been released up to that time since this is only 9% of the normal complement of mature eggs. By 4 hours, 50% of the populat
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
