. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ALBORAN SEA Pleurobrachia rhodopis Euplokamis stationis Undescribed cydippids. LOCATION OF SURVEY Non-luminous species Similar luminous species ASSAYS- Figure 1. Four types of assays were conducted to determine whether ctenophores were bioluminescent or bore any light-producing chemicals. At each site where specimens were collected, we also found luminous genera which could have been mistaken for Pleurobrachia. (P. bac/iei, fall). To ensure that the ctenophores were not prestimulated or damaged during collection, specimens u
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ALBORAN SEA Pleurobrachia rhodopis Euplokamis stationis Undescribed cydippids. LOCATION OF SURVEY Non-luminous species Similar luminous species ASSAYS- Figure 1. Four types of assays were conducted to determine whether ctenophores were bioluminescent or bore any light-producing chemicals. At each site where specimens were collected, we also found luminous genera which could have been mistaken for Pleurobrachia. (P. bac/iei, fall). To ensure that the ctenophores were not prestimulated or damaged during collection, specimens used in these studies were hand-collected in jars by blue- water divers, except at Friday Harbor, where they were collected from the surface in beakers. Because some cten- ophores lose their luminescence upon exposure to light (Ward and Seliger, 1976), specimens were dark-adapted fora minimum of 30 min prior to experiments. After this recovery period, Pleurobrachia were subjected to me- chanical, electrical, and a variety of chemical stimuli (Fig. 1). To ensure that the assay techniques were effective, we also tested the luminescence of other ctenophore species found at the same locations. Physical stimulation The most commonly applied test for luminescence was physical stimulation by a dark-adapted observer. This technique was used at all sites where ctenophores were collected. For quantitative tests of mechanical stimulation, five specimens of P. pileus collected in the Gulf of Maine (northwest Atlantic Ocean) were transferred to filtered seawater. allowed to dark-adapt, and stimulated by stirring in a photon-counting chamber for at least five seconds. This test was repeated three times with five or more P. bachei collected in the Santa Barbara Channel (eastern temperate Pacific Ocean). For comparison, luminous species were placed in the same apparatus and induced to luminesce by stirring or brief prodding. Because some organisms may be resistant to physical stimulation, ad- d
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
