. Pharmaceutical bacteriology. Bacteriology; Disinfection and disinfectants. BACTERIOLOGICAL TECHNIC "5 dish cover drop into place again; remove the cotton plug from test-tube by grasping it between two fingers (back of fingers toward the test-tube)- make the inoculation (deep stab, shallow stab, or streak); withdraw needle; replace cotton plug; hold needle in flame until glowing red. To prevent the sputtering of the material on the end of the needle, hold near flame until dry and then heat to redness. Singe free exposed end of the tube cotton plug in flame to kill and remove microbes and
. Pharmaceutical bacteriology. Bacteriology; Disinfection and disinfectants. BACTERIOLOGICAL TECHNIC "5 dish cover drop into place again; remove the cotton plug from test-tube by grasping it between two fingers (back of fingers toward the test-tube)- make the inoculation (deep stab, shallow stab, or streak); withdraw needle; replace cotton plug; hold needle in flame until glowing red. To prevent the sputtering of the material on the end of the needle, hold near flame until dry and then heat to redness. Singe free exposed end of the tube cotton plug in flame to kill and remove microbes and spores on the outer part of the cotton. The inoculated tubes are then numbered and incubated. In due time the cultural characteristics are noted and the observations entered in a suitable Pig. 41.—Turck ruling of the Thoma-Zeiss hemacytometer. This is especially useful if it is desired to combine the bacterial count with the spore and yeast count. The smaller areas (1-400 sq. mm.) may be used for making the bacterial counts, while the larger areas (1-25 sq. mm.) may be used for making the spore and yeast counts.— (Carl Zeiss.) Subcultures may also be made in Petri dishes, on potatoes, in tubes containing bouillon broth, blood serum, milk and other media with or without indicators. C. Studying Anaerobic Microbes.—Some microbes have anaerobic tend- encies (facultative aerobes) and some are absolutely anaerobic (obhgative anaerobes). The deep stab culture will show anaerobic tendencies. If such tendency exists, development will be more active near the bottom of tube (in the line of the stab). The culturing of obligadve anaerobes re- quires special apparatus though the methods are not in any way diflScult. The following methods are used: a. Deep stab culture. This has already been sufficiently explained. It merely indicates possible anaerobic tendencies. b. High-culture methods. Fill the tube of a deep stab culture, shallow stab or streak, with liquid agar or gel
Size: 1575px × 1586px
Photo credit: © The Book Worm / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorschneide, bookcentury1900, bookdecade1920, bookyear1920
