. Elements of applied microscopy. A text-book for beginners. Microscopy. ELEMENTS OF APPLIED MICROSCOPY. thus obtained, when placed in a cell, covered and ex- amined with the low objective, shows the spectrum of oxyhaemoglobin with dark bands in the yellow and green, shown in Fig. 50 at 4. If a drop of ammonium sulphide be added, the two bands will fuse into one, giving. 70 65 60 5,5 50 45 B C E 6 Fig. 50.—Spectra of Oxyhemoglobin and reduced Haemoglobin. (After Howell.) the spectrum of reduced haemoglobin, shown at B. The addition of a little citric acid to a solution of oxy- haemoglobin
. Elements of applied microscopy. A text-book for beginners. Microscopy. ELEMENTS OF APPLIED MICROSCOPY. thus obtained, when placed in a cell, covered and ex- amined with the low objective, shows the spectrum of oxyhaemoglobin with dark bands in the yellow and green, shown in Fig. 50 at 4. If a drop of ammonium sulphide be added, the two bands will fuse into one, giving. 70 65 60 5,5 50 45 B C E 6 Fig. 50.—Spectra of Oxyhemoglobin and reduced Haemoglobin. (After Howell.) the spectrum of reduced haemoglobin, shown at B. The addition of a little citric acid to a solution of oxy- haemoglobin produces the spectrum of acid haematin, with a broad band in the red, a narrow one in the green, and a very faint band in the blue. In old blood-stains the oxyhaemoglobin has been changed to haematin, and since this substance is insoluble in water, no color- ation of the liquid appears when the stain is treated as above. In such a case a Httle acetic acid must be tried; this dissolves hasmatin, and the solution at once pro- duces the acid haematin spectrum. On adding am- monia, the broad band in the red disappears. Blood- Stauas in which the haemoglobin is only partially changed. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Winslow, C. -E. A. (Charles-Edward Amory), 1877-1957. New York, J. Wiley; London, Chapman & Hall
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