. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Protein Stain Autoradiograph — 122KD — Stimulated o cL O ^c 00 (M C? CL IOO 200- 400- 600- 800- IOOO-. I200 LON LON Intact Cut t Stimulated Figure 4. The 122 kD protein is phosphorylated in vivo in the lateral eye in response to electrical stimulation of the efferent axons. This figure shows the results of a single assay (Table IB; animal 2, assay I). Early in the day, the LON was cut just anterior to one of the eyes. After the animal was placed in the apparatus to record ERG activity, the cut end of the LON that remained wi
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Protein Stain Autoradiograph — 122KD — Stimulated o cL O ^c 00 (M C? CL IOO 200- 400- 600- 800- IOOO-. I200 LON LON Intact Cut t Stimulated Figure 4. The 122 kD protein is phosphorylated in vivo in the lateral eye in response to electrical stimulation of the efferent axons. This figure shows the results of a single assay (Table IB; animal 2, assay I). Early in the day, the LON was cut just anterior to one of the eyes. After the animal was placed in the apparatus to record ERG activity, the cut end of the LON that remained with the eye was placed into a suction electrode. The animal was placed in the dark for 2 to 3 h. then the axons of the efferent neurons present in the LON were stimulated continuously with 15 V pulses (4 pps, 2 ms pulse duration) for 9 min (indicated by the arrow); the stimulation was then turned off for I min while the ERG amplitude was monitored. This sequence was repeated until the ERG amplitude reached a maximum, at which time both the stimulated and unstimulated eyes were removed and treated as described in Figure 3. As in Figure 3, the ERG amplitudes of both eyes were initially obscurred by voltage noise. In the lower record, the ERG amplitudes began to increase after the second interval of electrical stimulation. The ERG amplitudes remained small in the upper (control) record. The lower portion of this figure shows: the protein pattern visualized by silver stain (aliquots of solubi- lized preparations of both the stimulated and nonstimulated eyes contained about the same amount of 122 kD protein); an autoradiograph of a nitrocellulose blot containing larger (I0x) aliquots of these samples, subjected to the back phosphorylation procedure; the amount of 3:PO4 associated with the 122 kD protein; and the calculated amount of endogenous phosphory'ation of the protein in both samples. In the assay shown, we measured a increase in endogenous phosphorylation of the 122
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
