. The Biochemical journal. Biochemistry. PRECIPITATION OF ANTITOXIC SERA 289 from the unheated and from the heated sulphated plasmas were made and from the data thus obtained were calculated (a) the amount of protein remaining in every cc. of the filtrates, and (6) the respective precipitations of the serum proteins and of the antitoxin at each stage with the sulphate. It was found that in the unheated plasmas the preliminary treatment of the plasma affected the limits for the precipitation of the antitoxin-bearing proteins. Thus, in plasma of which the reaction lay between Ph7-4 and 8-3 the m
. The Biochemical journal. Biochemistry. PRECIPITATION OF ANTITOXIC SERA 289 from the unheated and from the heated sulphated plasmas were made and from the data thus obtained were calculated (a) the amount of protein remaining in every cc. of the filtrates, and (6) the respective precipitations of the serum proteins and of the antitoxin at each stage with the sulphate. It was found that in the unheated plasmas the preliminary treatment of the plasma affected the limits for the precipitation of the antitoxin-bearing proteins. Thus, in plasma of which the reaction lay between Ph7-4 and 8-3 the main part of the antitoxin-bearing proteins was precipitated in the Fi^tr. 7. Showing the relationship between the precii)itation of the antitoxin and of tlie serum proteins from cresylised plasma B by sodium sulphate 1001 \ 1 1 1 1 1 1 1 1 r—. 10 20 30 40 50 60 70 80 90 100 Percentage precipitation of the antitoxin with the protein precipitated at each stage with sodium sulphate The circumscribed j^oints © represent the data for the unheated plasma. The points at the intersection of two lines x represent the data for the heat- denaturated plasma The figures by the side of each point indicate the concentration of sodium sulphate in the plasma fraction isolated between 12 and 18-5 per cent, of sodium sulphate; from the same plasma to which 0-30 per cent, of cresylic acid had been added, the precipitation limits were respectively reduced to 11-5 and 16-5 per cent, of the suljjhate^. Furthermore, in the former case the limits were raised further by the dilution of the plasma; in the latter case they were lowered by an in- crease in the concentration of the cresylic acid in the plasma. 1 A similar lowering of the precipitation limits was seen in the precipitation of cresylised plasma by ammonium Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations ma
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Keywords: ., bookcentury1900, bookdecade1920, booksubjectbiochem, bookyear1920
