. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ARTIFICIAL MEDIA FOR ARTEMIA 443 amount to maintain a high growth rate? This led to inquiries on the role of particles, (see below) and to a practical solution: use of starch + or albumin. These advances were embodied in medium 91 which served for studies on replacing the particulate starch and precipitated protein with soluble carbon and nitrogen sources and for the determination of the purine-pyrimidine requirements. In Medium 100 (Table I) all nutrients aside from starch and albumin are denned. Mortality
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ARTIFICIAL MEDIA FOR ARTEMIA 443 amount to maintain a high growth rate? This led to inquiries on the role of particles, (see below) and to a practical solution: use of starch + or albumin. These advances were embodied in medium 91 which served for studies on replacing the particulate starch and precipitated protein with soluble carbon and nitrogen sources and for the determination of the purine-pyrimidine requirements. In Medium 100 (Table I) all nutrients aside from starch and albumin are denned. Mortality in this medium was low (15 instar) were obtained in 23 days after 2 transfer to 10 ml of new medium. Medium 100 still lacks the factors, supplied by liver infusion, for oogenesis. Without the addition of liver extract females carried eggs at 39 days but died at 45 days without depositing the eggs. With the addition of 75 mg% of liver extract females carrying eggs were obtained after 21 days and deposition after 25. 12 11 10 x OJ ~0 9 o 1_ O I I r. 10 20 30 Protein, m g % 40 50 ' 70B 90 60 80 FIGURE 2. Relationships between constant particulate starch (100 mg%) and varying quantities of precipitated proteins. O = precipitated proteins (albumin 6 p + 7 globulin 2 p + /3 lactoglobulin 2 p) ; exper. 152; 10 ml medium 91 (no amino acids); 5 animals; 12 days growth. A = 7-globulin; exper. 152, same conditions. D = ^-lactoglobulin; exper. 152, same conditions. O = egg albumin; exper. 180; 10 ml medium 100 (no amino acids); 5 animals; 12 days growth. Growth index, see Material and Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Ca
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