. The eggs of mammals. Ovum; Embryology -- Mammals; Mammals -- physiology; Ovum. Yamane (1930) has ascribed the phenomenon of folHcle cell dispersion to the presence of a proteolytic enzyme in the spermatozoa. He was able to secure a similar dispersal of follicle cells from sperm sus- pensions heated to 60° C. and from preparations of pancrea- tin containing trypsin. Yam- ane (1930) believes that this J "^ '^^^^P^^^' J^^H proteolytic enzyme is also re- f f^&^S^X€jli^S sponsible for the activation of the egg since he observed ''polar" bodies formed in rabbit ova exposed to the sus
. The eggs of mammals. Ovum; Embryology -- Mammals; Mammals -- physiology; Ovum. Yamane (1930) has ascribed the phenomenon of folHcle cell dispersion to the presence of a proteolytic enzyme in the spermatozoa. He was able to secure a similar dispersal of follicle cells from sperm sus- pensions heated to 60° C. and from preparations of pancrea- tin containing trypsin. Yam- ane (1930) believes that this J "^ '^^^^P^^^' J^^H proteolytic enzyme is also re- f f^&^S^X€jli^S sponsible for the activation of the egg since he observed ''polar" bodies formed in rabbit ova exposed to the suspensions of dead sperm and to the enzyme prepara- tions. Pincus and Enzmann (1935) have examined this situation in some detail. Sperm suspensions free of seminal fluid were obtained from the vas deferens of adult rabbit males. Dilutions were made with a buffered Ringer- Locke solution at pH — The ova were taken at 12 H to 153^ hours after copulation from rabbit does mated to sterile (vasecto- mized) males; these ova were invariably well embedded in the massed follicle cells. The procedure followed was to place the massed ova in the sperm suspension and incubate for at least two hours. All ova were examined at two hours after semination and in some instances where no obvious signs of fertilization were observed incubated for 12 hours. Fig. 24. Rat ovum of Fig. 23 after 2 hours with Hving sperm. Note absence of folhcle cells and protrusion resembling a polar body. (From the Aiia- tomical Record.). Fig. 25. ovum recovered 11 hours after sterile mating and in- cubated with living sperm for 2 hours. Note shrunken vitellus and two polar bodies. (From the Ana- tomical Record.). Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Pincus, Gregory, 1903-1967. New York, The Macmillan Company
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