. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HEMOCYANIN SUBUNITS IN ARTHROPODS 95 Hydrachno geographica g(4] Mastigoproctus brasilianus Tnchodamon froesi Tarantuta palmata Androctonus australis Leiurus quinquestriatus Colossendeis spec Polyphemus Tachypleus tndentatus. Eurypelma Androctonus Colossendeis —' Cupienmus SDS PAGE FIGURE 2. Distribution of hemocyanin among the subphylum Chelicerata. The patterns of crossed immunoelectrophoresis show the respective subunit composition; hatched squares symbolize the native aggregation states (8 X 6,4 X 6, 2 X 6; small
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HEMOCYANIN SUBUNITS IN ARTHROPODS 95 Hydrachno geographica g(4] Mastigoproctus brasilianus Tnchodamon froesi Tarantuta palmata Androctonus australis Leiurus quinquestriatus Colossendeis spec Polyphemus Tachypleus tndentatus. Eurypelma Androctonus Colossendeis —' Cupienmus SDS PAGE FIGURE 2. Distribution of hemocyanin among the subphylum Chelicerata. The patterns of crossed immunoelectrophoresis show the respective subunit composition; hatched squares symbolize the native aggregation states (8 X 6,4 X 6, 2 X 6; small squares correspond to hexamers *). Immunologically homologous subunits are identically labeled using the designations a-h established for spider hemocyanins. Scorpion and xiphosuran subunits are additionally labeled according to their original designations (Lamy et ai, 1979a, b, c; Markl, 1980). The results from Tachypleus and Leiurus (Lamy et ai, 1979c; Markl et ai. 1984) are not discussed in the text, but fit well into the scheme. Whip spider and whip scorpion hemocyanin subunits were studied only by electrophoresis (Markl et ai, 1979b). Opilionids possess 2x6 hemocyanin (Kempter et ai, 1985), whereas no hemocyanin was found in Galeodes, Hydrachna, and Colossendeis (Markl et ai, 1986a). The figure is taken from Markl et ai (1986a). Left insert: polyacrylamide gel electrophoresis (PAGE) of hemolymph proteins in the presence of a detergent (sodium dodecyl sulphate = SDS), showing that the sea spider Colossendeis possesses no hemo- cyanin (He), but it does possess the typical arachnidan non-respiratory protein (NRP). This was con- firmed by immuno blotting. Comparable unpublished results were recently obtained with the pycnogonid Nymphon sp. Right insert: phylogenetic relationships between the eight cheliceratan subunits according to the combined information of Lamy et ai, 1983a, Markl et ai, 1984, and Kempter et ai, 1985. Broader lines indicate that with anti-crustacean alpha
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
