. Ciba Foundation symposium on drug resistance in micro-organisms: mechanisms of development. Drug resistance in microorganisms. Mutagenic Alkylating Agents But on esterification no acid is released: 301 >NCH2CH2C1+ = P0-Na >NCH2CH2 0P=+NaCl. With relatively dilute solutions of DNA a considerable amount of acid is produced during the reaction with the 100 80 ^60 o o 40 o 20 'NoOOIN-NaCI ONLY \ CORRESPONDS TO 35 70 OF PHOSPHATE GROUPS ESTERIFIED. SO 100 150 200 250 TIME (mIN) 300 350 400 Fig. 2. Rate of acid formation when the phenylalanine nitrogen mustard at a concentration of 0 • 1 % w
. Ciba Foundation symposium on drug resistance in micro-organisms: mechanisms of development. Drug resistance in microorganisms. Mutagenic Alkylating Agents But on esterification no acid is released: 301 >NCH2CH2C1+ = P0-Na >NCH2CH2 0P=+NaCl. With relatively dilute solutions of DNA a considerable amount of acid is produced during the reaction with the 100 80 ^60 o o 40 o 20 'NoOOIN-NaCI ONLY \ CORRESPONDS TO 35 70 OF PHOSPHATE GROUPS ESTERIFIED. SO 100 150 200 250 TIME (mIN) 300 350 400 Fig. 2. Rate of acid formation when the phenylalanine nitrogen mustard at a concentration of 0 • 1 % was reacted with different con- centrations of DNA at 37° C (pH maintained at 7 by an automatic titrator), and the effect of heating subsequently for 20 minutes at 90° C. mustard. That the liberation of this acid is entirely due to hydrolysis by water and not produced in part by alkylation of amino groups was shown by analysis of the solution after reaction was complete. The amount of mustard which had combined with the DNA was equal to that bound by esteri- fication as calculated from the decrease in the amount of acid released, while the amount of hydrolysed mustard found. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ciba Foundation; Wolstenholme, G. E. W. (Gordon Ethelbert Ward); O'Connor, Cecilia M. (Cecilia Mary), 1927-. Boston, Little, Brown
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