. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. s p cp cp p s FIGURE 1. Electrophoresis on continuous gradient gels of the crayfish hemolymph, in tris borate EDTA buffer pH Serum is represented by (s) ; plasma (p) ; and clottable protein (cp). FIGURE 2. Sodium dodecyl sulfate electrophoresis after incubation in SDS and urea presence. Gel was run in phosphate buffer containing SDS for 15 hours with 15 mAmp and 30 V. Symbols are as in Figure 1. pared in distilled water, no clot was formed. It seems that this anti-coagulant chelates the calcium necessary for the tr
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. s p cp cp p s FIGURE 1. Electrophoresis on continuous gradient gels of the crayfish hemolymph, in tris borate EDTA buffer pH Serum is represented by (s) ; plasma (p) ; and clottable protein (cp). FIGURE 2. Sodium dodecyl sulfate electrophoresis after incubation in SDS and urea presence. Gel was run in phosphate buffer containing SDS for 15 hours with 15 mAmp and 30 V. Symbols are as in Figure 1. pared in distilled water, no clot was formed. It seems that this anti-coagulant chelates the calcium necessary for the transformation of the clottable protein into gel and also irreversibly blocks the conversion. The solutions of sodium citrate or potassium oxalate do not block the hemolymph clotting in the same way. With sodium citrate, univalent ions were necessary to permit the reversibility of this reaction. It was noted that the pH values of sodium citrate in distilled water or in physiological saline were almost identical. However when univalent ions were added in the citrate solution, before the mixture with hemolymph, the clotting appeared easily ( ml of blood was with- drawn on 100 M sodium citrate + 100 /A! M NaCl or KC1 and clotted in 15 minutes following the addition of 100 /J M CaCl2). Plasmatic clottable protein. When obtained from oxalated plasma, it clotted very strongly within 10 minutes ( ml clottable fraction + 50 [A M CaClo + 50 fA cellular extract) ; but it failed to elicit a clotting response when it was extracted from citrated blood, by the same salting out procedure. However, with a citrated plasma containing Na+Cl~ ions, the extraction furnished a solution which clotted perfectly. On the polyacrylamide gradient gels, no differences were noted between the electrophoretic patterns of active and inactive preparations. Clotting tests per- formed with clottable protein reconstituted after storage in (NH4)2 SO^ wrere uniformly negative, but freezing s
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
