. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 438 H. SUGITA AND K. SEKIGUCHI A B. Cr Tt Tg Cr rt tr Tt gt Tg FIGURE 1. Electrophoretic patterns of horseshoe crab LDH from hepatopancreas tissues (A) and from the first-instar larvae (B). Vertical starch gel electrophoresis was carried out at 4°C with 12% gel, using the buffer system of Selander and Yang (1969. Gel buffer: M Tris and M citric acid, pH Electrode buffer: M boric acid and M NaOH, pH ). Cr = Carcinoscorpius rotundicauda; Tt = Tachypleus tridentatus; Tg = Tachypleus gigas; rt = hybri
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 438 H. SUGITA AND K. SEKIGUCHI A B. Cr Tt Tg Cr rt tr Tt gt Tg FIGURE 1. Electrophoretic patterns of horseshoe crab LDH from hepatopancreas tissues (A) and from the first-instar larvae (B). Vertical starch gel electrophoresis was carried out at 4°C with 12% gel, using the buffer system of Selander and Yang (1969. Gel buffer: M Tris and M citric acid, pH Electrode buffer: M boric acid and M NaOH, pH ). Cr = Carcinoscorpius rotundicauda; Tt = Tachypleus tridentatus; Tg = Tachypleus gigas; rt = hybrid between Cr 9 and Tt S; tr = hybrid between Tt 9 and Cr <J; gt = hybrid between Tg 9 and Tt <?. was polymorphic (Sugita and Sekiguchi, in prep.). Genetic variants of the LDH could not be detected in larvae developed from eggs of a single female. In hybrid progeny obtained from the interspecific crosses of all 6 combinations among 3 species as well as in normal progeny from the control crosses, the early embryos displayed only the maternal LDH pattern which could be detected in unfertilized eggs (results not shown). The maternal LDH from these embryos had similar relative mobility to the LDH from the first-instar larvae (Fig. 1 B). In hybrid embryos between T. tridentatus 9 and C. rotundicauda 6, the paternal form of the LDH was first detected at stage 17 (30 days after insemination, im- mediately before the 1st embryonic molt) (Fig. 2A), but the paternal form of the LDH was not observed even on the 41st day after insemination (stage 19, after the the 2nd embryonic molt) in hybrid embryos between C. rotundicauda 9 and T. tridentatus 3. The LDH from hybrid embryo between T. gigas 9 and T. tridentatus 6 occurred in 3 molecular forms at stage 20 (32 days after insemination, after the 3rd embryonic molt), suggesting that the LDH of the hybrid embryo consisted of a maternal hom- odimer, a paternal homodimer, and a hybrid heterodimer (Fig. 2B). This paternal form of the enzyme
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