. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AMEBOCYTE AGGREGATION IN LLMULUS 551 100 A 90- 80- Z o en ^ 70 5 c/) z a 60- z LU 0 cr LU CL 50- 40- 30 -»--. /vtfYiftww^^ B 0 2 TIME 4 (min) FIGURE 1. Typical photometric record of amebocyte aggregation at standard conditions. Physiological saline at 15° C is the diluent and stirring speed is 300-335 rpm. Records of aggregation show reproducible characteristics; decrease in transmission resulting from addi- tion of amebocytes to the cuvette CA-B) ; a lag period (B-C) ; rapid rise in transmission (C-D) ; levelling off period
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AMEBOCYTE AGGREGATION IN LLMULUS 551 100 A 90- 80- Z o en ^ 70 5 c/) z a 60- z LU 0 cr LU CL 50- 40- 30 -»--. /vtfYiftww^^ B 0 2 TIME 4 (min) FIGURE 1. Typical photometric record of amebocyte aggregation at standard conditions. Physiological saline at 15° C is the diluent and stirring speed is 300-335 rpm. Records of aggregation show reproducible characteristics; decrease in transmission resulting from addi- tion of amebocytes to the cuvette CA-B) ; a lag period (B-C) ; rapid rise in transmission (C-D) ; levelling off period (D-E) ; a plateau period (E-F). When transmission is converted to and aggregation is expressed as per cent change in , the resulting curve has the same shape as that shown above. ml cold saline and then frozen in ml saline. After thawing, the pellet was homogenized by rotary motor at 1000 rpm or by hand for 5 min employing a ground glass homogenizer and teflon pestle. Freeze-thawing alternated with homogenization was repeated twice more. During homogenization, the homogenate was kept on ice and precautions taken to prevent foaming. The final volume of the homogenate was 2 ml, which represents approximately a 5-fold concentration of amebocytes in the original volume of hemolymph. The amebocyte homogenate was then centrifuged at 0° C at 12,100 X g for ten minutes. After centrifugation, the slightly opaque supernatant was drawn off and examined under a phase micro- scope to make certain that cellular fragments were absent. The AHS yield was ml for each 2 ml of homogenate and ml aliquots were stored at -20° Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/0
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
