. Biological structure and function; proceedings. Biochemistry; Cytology. 54 F. EDMUND HUNTER, JR. values, much lower than the plateau seen with phosphate and substrates. An important characteristic is that ascorbate lysis occurs only with low concentrations, o-2-i mM being optimal. Higher concentrations lengthen 0,5 0,3 CONTROL -â¢-â -â¦â* "--a. â¢^ ASCORBATE 5mM ^. lOmM. i 5m M PO4 2mM^-0H-BUTYRATE \ A ImM ASCORBATE ^ 20 30 40 MINUTES 50 60 Fig. I. Absorbancy changes at 520 m/it when dilute suspensions of liver mitochondria are treated with phosphate, /3-hydroxybutyrate
. Biological structure and function; proceedings. Biochemistry; Cytology. 54 F. EDMUND HUNTER, JR. values, much lower than the plateau seen with phosphate and substrates. An important characteristic is that ascorbate lysis occurs only with low concentrations, o-2-i mM being optimal. Higher concentrations lengthen 0,5 0,3 CONTROL -â¢-â -â¦â* "--a. â¢^ ASCORBATE 5mM ^. lOmM. i 5m M PO4 2mM^-0H-BUTYRATE \ A ImM ASCORBATE ^ 20 30 40 MINUTES 50 60 Fig. I. Absorbancy changes at 520 m/it when dilute suspensions of liver mitochondria are treated with phosphate, /3-hydroxybutyrate, or ascorbate. All additions made at zero time. Mitochondrial protein was 150 /^ o in - - -» ⢠^. CONTROL v^»\r^ ⢠â « '\v\ \ \ \\ \ \« \ ^ 5mM GSSG gsh\^ *^ \^ 2mM-''\ ^ ⢠4 \ \ \ \\ \ 1 ^ \ 5mM \ VlOmM \ yK l5mM \ ' \r i \ 5mM \ V \^ VCYSTEINE x^^ â ⢠1 1 ⢠⢠L â"" â ' " * 10 20 30 40 Ml NUTES 50 60 Fig. 2. Absorbancy changes due to swelling or lysis of mitochondria induced by cysteine or glutathione. the lag period and inhibit swelling, until 15 mM prevents swelling com- pletely for I to 2 hr. This has special significance, for high concentrations of ascorbate do not block phosphate or substrate-induced swelling. Figure 2 demonstrates that reduced glutathione (GSH) and cysteine produce an absorbancy change like that seen with low concentrations of. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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