Physiology and biochemistry in modern medicine . yringeshould be moved backward and forward several times, but it must not bshaken. After centrifuging, about 3 of plasma are removed and saturatedwith CO, at the same tension as in alveolar air (i. e., per cent). This 44 PHYSICOCHEMICAL BASIS OF PHYSIOLOGICAL PROCESSES is done by placing the plasma in a separating funnel of 300 capacity,laying the funnel on its side and displacing the air in it by alveolar airsecured by quickly making as deep an inspiration as possible throughthe tube and bottle containing glass beads (Fig. 10). Th
Physiology and biochemistry in modern medicine . yringeshould be moved backward and forward several times, but it must not bshaken. After centrifuging, about 3 of plasma are removed and saturatedwith CO, at the same tension as in alveolar air (i. e., per cent). This 44 PHYSICOCHEMICAL BASIS OF PHYSIOLOGICAL PROCESSES is done by placing the plasma in a separating funnel of 300 capacity,laying the funnel on its side and displacing the air in it by alveolar airsecured by quickly making as deep an inspiration as possible throughthe tube and bottle containing glass beads (Fig. 10). The glass beadsremove excess of water vapor from the air. The funnel must be restop-pered before the end of the expiration, so that no outside air enters. Itis then rotated, for about two minutes, in such a way that the plasmaforms a film on its walls. If it is necessary to postpone the saturatingof the plasma, this should be pipetted off from the corpuscles and pre-served in hard glass test tubes coated with paraffin. From ordinary glass. Fig. 11.—Van Slykes apparatus for measuring the CO^-combining power of blood in blood description, see context. enough alkali is soon dissolved out to vitiate the results. After saturationof the plasma with C02, the funnel is placed in the upright position andthe plasma allowed to collect in the narrow portion, after which 1 removed with an accurate pipette and analyzed for C02. The analysis may be done by using either the Van Slyke or the Hal-dane-Barcroft apparatus. The Van Slyke method is as follows: The apparatus is filled to the top of the graduated tube with mercury(Fig. 11) by raising the mercury reservoir F, care being taken thatD and E are also filled. One of the C02-saturated plasma is then de- ACIDOSIS 45 livered into A (which has been rinsed out with C02-free ammonia water),and the stopcock / turned so that by cautiously lowering the level of thereservoir F, the plasma runs into B (but no trace of air). The
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