. Carnegie Institution of Washington publication. WILT OF CUCURBITS. 223 (15.) The pricked leaf was the first to show signs of the disease. They were noted the eleventh day after inoculation, but as the droop did not seem to proceed from any particular spot I was in doubt as to its cause. The thirteenth day the leaf above and the one below the inoculated leaf showed wilt. The fourteenth day they began to shrivel, the whole blade of the inoculated leaf was dry- shriveled, three additional leaves farther up the stem also showed a decided droop, and a fourth one, still higher up, a slight flabbin
. Carnegie Institution of Washington publication. WILT OF CUCURBITS. 223 (15.) The pricked leaf was the first to show signs of the disease. They were noted the eleventh day after inoculation, but as the droop did not seem to proceed from any particular spot I was in doubt as to its cause. The thirteenth day the leaf above and the one below the inoculated leaf showed wilt. The fourteenth day they began to shrivel, the whole blade of the inoculated leaf was dry- shriveled, three additional leaves farther up the stem also showed a decided droop, and a fourth one, still higher up, a slight flabbiness. The rest of the leaves above and below were turgid and showed no sign of the wilt. The disease was moving up faster than down, as in some cases previously recorded. The following day (October 4) three additional leaves nearer the tip were wilted and one more toward the base making eleven in all, to wit: eight above the inoculated leaf and two immediately below it. The remaining basal leaf and the four leaves at the tip of the vine were still turgid. Two of the four noted as having wilted the previous day (the two nearest the point of infection) were then shriveling. The plant was now brought into the laboratory. The seventeenth day the wilt showed on the lowest leaf. All the leaves farther up as well as the stem in places had begun to shrivel. When segments of the stem were examined microscopically, the vessels were found to be full of the bacteria, which varied in size noticeably and looked much larger than usual (involution forms?). In the primary vessel parenchyma were many destructive lesions. The bacillus was also found in a small, green fruit, hanging midway on the stem and looking sound externally. Here it was confined to the bundles in the outer ring from which it slowly oozed on cross-section. The next day (October 7) the cut surface over the affected bundles was covered with a milky and very viscid bacteria] slime which strung out on the tip of a needle a distanc
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