. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. UPTAKE OF AMINO ACIDS BY PLATYMONAS 395 TABLE II Uptake rates of glycine at three cell concentrations. Cells were exposed to 7 X 10~7 M glycine-Cu for 30 minutes Cell concentration (number per mlj Ratio Uptake rate (cpm/min.) Ratio 3 X 105 1 90 1 1 X 106 270 X 106 775 of glycine-C14 continued at concentrations as low as 5 X 10 * molar; a suspension of 106 cells per ml. removed 96% of the radioactivity after 60 minutes. Assimilation Cells incubated with glycine-C14 were collected at intervals and extracted
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. UPTAKE OF AMINO ACIDS BY PLATYMONAS 395 TABLE II Uptake rates of glycine at three cell concentrations. Cells were exposed to 7 X 10~7 M glycine-Cu for 30 minutes Cell concentration (number per mlj Ratio Uptake rate (cpm/min.) Ratio 3 X 105 1 90 1 1 X 106 270 X 106 775 of glycine-C14 continued at concentrations as low as 5 X 10 * molar; a suspension of 106 cells per ml. removed 96% of the radioactivity after 60 minutes. Assimilation Cells incubated with glycine-C14 were collected at intervals and extracted with 80% ethanol. The extract was evaporated to dry ness and the residue dissolved in isopropanol. Ten to 20 microliters of this solution were spotted on Whatman filter paper. An ascending separation was carried out in 2 dimensions with the solvent systems: butanol, acetic acid, water, 12:3:5, v/v/v; phenol, water 18:5, w/v. Autoradiograms (Kodak "No-Screen" medical x-ray film) of resulting 35 30 025 X < a. D 2X10 M 1X10 M 9 5 10 M *. 10 IS 20 IS 30 35 40 TIME (minutes) FIGURE 3. Uptake of glycine-C14 at 4 concentrations. Points are 1-inl. samples containing 5 X 105 cells. Lines obtained with the least squares method. [Q] indicates decrease in uptake rate due to depletion of glycine in the Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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