. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 210 R. J. ET AL. a Kn- ot 2 (I)'. Figure 1. SDS-PAGE of(A) type I collagen, and (B) the total insoluble matrix of Lci>t»f!<»'Kia \'iri;iiltitu. Also present are molecular weight components equal to the ft and y chains of type I collagen. A CNBr digest of the solubilized collagen from the July insoluble matrix showed, on 3-17% acrylamide gradient gels, different patterns from that of a CNBr digest of type I collagen (Fig. 2). Treatment with urea did not change the pattern of the insoluble matrix. The total ins
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 210 R. J. ET AL. a Kn- ot 2 (I)'. Figure 1. SDS-PAGE of(A) type I collagen, and (B) the total insoluble matrix of Lci>t»f!<»'Kia \'iri;iiltitu. Also present are molecular weight components equal to the ft and y chains of type I collagen. A CNBr digest of the solubilized collagen from the July insoluble matrix showed, on 3-17% acrylamide gradient gels, different patterns from that of a CNBr digest of type I collagen (Fig. 2). Treatment with urea did not change the pattern of the insoluble matrix. The total insoluble matrix apparently did not enter the gel and therefore re- mained uncleaved by CNBr. SDS-PAGE of the matrix not solubilized with pepsin revealed low molecular weight proteins that stained with PAS (Fig. 3), indicating the presence of glycoproteins in this matrix. Following the NaB3H4 reduction of the insoluble ma- trix, cross-link analysis indicated that the only reduced cross-link present was DHLNL (Fig. 4). Each nmole of collagen contained nmoles of DHLNL. No stable non-reducible cross-links were detected. Discussion This report is the first to demonstrate conclusively the presence of a "classic" collagen ( a protein containing Hyp, Hyl, and 33% Gly as well as a typical collagen cross- link) as part of the organic matrix of a calcite (calcium carbonate) invertebrate skeletal structure. The insoluble organic matrices from calcite spicules of Leptogorgia vir- gulata collected in the summer months have amino acid compositions characteristic of collagen (Table IA, C). Those compositions are similar to other soft tissue coel- enterate collagens (Franc, 1985). When the amino acid composition is viewed in toto. strong similarities to the pepsin soluble fraction, and sea anemone and vertebrate collagen are evident (see Table I C, E, I, J). The SDS- PAGE pattern of the July insoluble matrix (after pepsin digestion) also displayed the characteristic of type
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
