. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 202 REED A. FLICKINGER reacted with a % protein supernate from adults' brains at an antiserum dilution of 1/128. Obviously extracts of adult frog brains are not highly antigenic. In the preparation of test antigens, early gastrulae (Shumway stage 10) were operated upon under sterile conditions and cut in four regions; ectoderm, dorsal mesoderm, ventral mesoderm and endoderm, as seen in Figure 1. Most of the large white yolky cells were removed from the ventral and dorsal mesoderm tissues. Recently hatched larvae (Shumway
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 202 REED A. FLICKINGER reacted with a % protein supernate from adults' brains at an antiserum dilution of 1/128. Obviously extracts of adult frog brains are not highly antigenic. In the preparation of test antigens, early gastrulae (Shumway stage 10) were operated upon under sterile conditions and cut in four regions; ectoderm, dorsal mesoderm, ventral mesoderm and endoderm, as seen in Figure 1. Most of the large white yolky cells were removed from the ventral and dorsal mesoderm tissues. Recently hatched larvae (Shumway stage 19-20) were cut into three parts; head, trunk and gut (Fig. 1). The heads were removed by cutting verti- ECTODERM HEAD TRUNK VENTRAL MESODERM. DORSAL MESODERM ENDODERM. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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