. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MORPHOLOGICAL COLOR CHANGE IN CRUSTACEA 241 As a further check on the incorporation of C14 into the black pigment, crabs were injected with /xC. of L-valine-1-C1* and with of glycine-1-C1*. After 24 hours on neutral background with natural lighting no significant levels of radioactivity could be found in the pigment extracted from crabs injected with either amino acid. Concentration curve Forty crabs, in groups of four, received varying amounts of DL-tyrosine-2-C14. After 24 hours on a neutral background with na
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MORPHOLOGICAL COLOR CHANGE IN CRUSTACEA 241 As a further check on the incorporation of C14 into the black pigment, crabs were injected with /xC. of L-valine-1-C1* and with of glycine-1-C1*. After 24 hours on neutral background with natural lighting no significant levels of radioactivity could be found in the pigment extracted from crabs injected with either amino acid. Concentration curve Forty crabs, in groups of four, received varying amounts of DL-tyrosine-2-C14. After 24 hours on a neutral background with natural lighting, the area of the carapace was determined and the pigment extracted from the epidermis. Interpo- C\J or Ld LU h- 800- 700-i 600- I sooH cr £400- I 300- O 200- I 00- n. A A 40 80 20 160 200 14 MICROGRAMS TYROSINE-C PER GRAM WET WEIGHT FIGURE 1. The relationship between the counts per minute per square centimeter of cara- pace of the extracted pigment and micrograms DL-tyrosine-2-C" injected per gram wet weight. The linear regression line has been calculated and the P — confidence interval of the slo is given. Y = + ^ b ^ P < Solid dots = ^C.; O = MC.: A = ; H = (; V = jtC.: microcuries injected per animal. lation from a standard curve relating the area of the carapace to the wet weight of dechelate male crabs gave the total body weight. On this basis the /xg. of tyrosine-C1* injected per gram wet weight of the crabs was calculated. A regres- sion analysis of /*g. tyrosine injected per gram wet weight against counts per minute per square centimeter of carapace of extracted pigment was Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Labora
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
