. Pathological technique; a practical manual for workers in pathological histology and bacteriology. d as Type IV pneumococcus. Asall strains of pneumococcus are dissolved by bile and allstrains of streptococci are not, the fifth tube serves to pre-vent the mistake of classifying a streptococcus as Type IVpneumococcus. The bile is prepared by autoclaving, filter-ing off the precipitate, and again autoclaving. Types I and II occur in about equal proportion in morethan 60 per cent, of all the cases of pneumonia investigated. Type III is apparently identical with some of the strainsof Streptococc
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. d as Type IV pneumococcus. Asall strains of pneumococcus are dissolved by bile and allstrains of streptococci are not, the fifth tube serves to pre-vent the mistake of classifying a streptococcus as Type IVpneumococcus. The bile is prepared by autoclaving, filter-ing off the precipitate, and again autoclaving. Types I and II occur in about equal proportion in morethan 60 per cent, of all the cases of pneumonia investigated. Type III is apparently identical with some of the strainsof Streptococcus Capsulatus, the characters of which wedescribe elsewhere (see page 270), and occurs only in asmall percentage of cases. 270 PATHOLOGICAL TECHNIQUE. Type IV occurs in about 20 per cent. For control, cultures on blood-agar plate and in bouillonshould be made from the heart blood of the mouse, and witha pure bouillon culture thus obtained confirmation of thetype should be carried out as in the case of the bacterialsuspension described above. If a positive culture from the blood of the patient be. FIG. 43- Fig. 44- Fig. 45- Fig. 43.—Pneumococcus; blood-serum 44.—Streptococcus capsulatus ; blood-serum 45-—Streptococcus capsulatus; glucose-agar stab culture (Oscar Rich-ardson ; photos, by L. S. Brown). available, 10 of the fluid should be centrifugated and asuspension of the organism prepared and tested as describedabove. Likewise the determination of type may be madefor pneumococci obtained by culture from spinal fluids andother material. Streptococcus Capsulatus.—This seems to be the bestname to apply to a capsule-bearing Gram-positive bac-terium resembling both the pneumococcus and the strepto- PATHOGENIC BACTERIA AND FUNGI. 2*}\ coccus pyogenes in morphology, but differing definitely fromthem in cultural and other peculiarities. It has been foundchiefly in lobar pneumonia, but occurs in other inflammatoryprocesses and probably has been so
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