. Bacteria in relation to plant diseases. Bacteria; Plant diseases. Fig. 66.* gas was formed. Its color on potato was at first pale yellow and then distinctly ochraceous a color lying between ochraceous and ochre-yellow (Ridgway's plate v, 7 and 9), i. e., there was much more buff in it than in cultures of Bad. campestre. It was cultivated at room temperatures ranging from 180 to 240 C. The substratum was soon stained a de- cided gray (not brown), drab-gray (Ridgway's plate 11, 13). I have a note saying that several tubes of potato inoculated with Bacterium campestre the same day were not gray
. Bacteria in relation to plant diseases. Bacteria; Plant diseases. Fig. 66.* gas was formed. Its color on potato was at first pale yellow and then distinctly ochraceous a color lying between ochraceous and ochre-yellow (Ridgway's plate v, 7 and 9), i. e., there was much more buff in it than in cultures of Bad. campestre. It was cultivated at room temperatures ranging from 180 to 240 C. The substratum was soon stained a de- cided gray (not brown), drab-gray (Ridgway's plate 11, 13). I have a note saying that several tubes of potato inoculated with Bacterium campestre the same day were not grayed (fourth day). At the end of 25 days a little of the potato from immediately under the thin layer of bacteria blued litmus paper decid- edly. On mashing old potato cul- tures in iodine potassium-iodide water there was a copious brown-pur- ple reaction, showing that the starch had been acted upon only a little. The organism grew on sugar- beet cylinders, producing consider- able yellow slime, but the culture was dead at the end of 5 months. In its morphology, so far as examined, it closely resembled the ordinary forms of Bad. hyacinthi, being a short rod with rounded ends. No long chains, filaments or endospores were observed. Its general morphology and relation to the tissues is shown in figures 65, 66, and 67. So far as I have been able to deter- mine from an inspection of the microtome sections the vessels are occupied (a small number only in the material cut), the inter- cellular spaces in the pith (frequently), and also occasionally large pith-cells. The latter have pits in their walls, and appar- ently the bacteria have forced their way through these thin places into the interior of these cells. Surrounded by free cells these bacterially occluded cells present a striking appearance when stained deep red with fuchsin. The bacteria were found fre- quently in crystal-cells. I have observed no bacteria in the cortex, which is made up largely of collenchyma. Fig. *Fig. 6
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