. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 216 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS. MBP MtO Know n EST Unknow n Distribution of cDNA sequences Figure 1. Number and distribution ofcDNAs obtained from subtractive hybridization studies with rat brain samples homogenized in M sucrose (black bars) or M sucrose (gra\ bars). Clearly larger numbers of novel (30 vs. 13) cDNAs, many of which are related to known mRNAs (see Table I}, are obtained in the sample homogenized in M sucrose. This difference is mainly due to the higher numbers (58 vs. 33) o
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 216 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS. MBP MtO Know n EST Unknow n Distribution of cDNA sequences Figure 1. Number and distribution ofcDNAs obtained from subtractive hybridization studies with rat brain samples homogenized in M sucrose (black bars) or M sucrose (gra\ bars). Clearly larger numbers of novel (30 vs. 13) cDNAs, many of which are related to known mRNAs (see Table I}, are obtained in the sample homogenized in M sucrose. This difference is mainly due to the higher numbers (58 vs. 33) of cDNAs derived from niYc/in basic protein (MBP) or myelin-associated oligodendrocytic basic protein (MOBP) mRNAs in the M sucrose sample. Other abbreviations: Mito, sequences related to the mitochondria! genome: Known, sequences in the non-redundant GenBank database: KR, sequences related to known GenBank sequences but with less than 50% of the set/uence matching the known: EST, sequences in the GenBank EST database; Unknown, sequences unrelated to sequences in either the non-redundant or EST databases. The results of this study will, in particular, help us to select appropriate conditions to homogenize dogfish brain, which exists in an environment of far higher tonicity (approximately I M) than mammalian brain. This work was funded by the National Multiple Sclerosis Soci- ety grant RG2944 (RMG) and the G. Linger Vetlesen Foundation (HGM). Funds for John Engler were from the Marine Models in Biological Research Proaram (NSF grant, DBI-9912287). We Identities of "known" mRNAs obtained in this study Table 1 Name Accession* Size (region)t Homogenization Astrocytic phosphoprotein AJ243949 1565-2073(2341) M Phosphodiesterase I D28560 2282-2898(3216) M Ferritin light chain NM_008064 605-886(886)** M Glial maturation factor NMJI04124 6-291 (4131) M Eukaryotic translation elongation factor « 1 X63561 584-1449(1714) M Eukaryotic translation elon
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