. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 278 SHIRO IIORIUCHI AM) CHARLES E. LANE (Fig. 5). Activity was unditnini-Ucl up to 45° C. Between 45° C. and 50° cellukilytic activity was markedly reduced and was 90% destroyed at 70° C. Cellobiase activity (Table EV) was estimated by incubating cellobiose with crystalline style en/.yme solul llucose produced was determined by the spe- cific glucose oxidase method \Yorthington Biochemical Corp.). As compared with the cellulase activity, the cellobiase acthity is slight. Since the crystalline style of -V. gigas appears i
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 278 SHIRO IIORIUCHI AM) CHARLES E. LANE (Fig. 5). Activity was unditnini-Ucl up to 45° C. Between 45° C. and 50° cellukilytic activity was markedly reduced and was 90% destroyed at 70° C. Cellobiase activity (Table EV) was estimated by incubating cellobiose with crystalline style en/.yme solul llucose produced was determined by the spe- cific glucose oxidase method \Yorthington Biochemical Corp.). As compared with the cellulase activity, the cellobiase acthity is slight. Since the crystalline style of -V. gigas appears i deficient in cellobiase, it appears that digestion of cellulose by this animal does not necessarily include cellobiose as an intermediate (Levinson and Reese. 1"50). — 200 LU CO 8 CO a: % CO o o 100 0. 20 30 40 TEMPERATURE °C 50 FIGURE 4. Effect of temperature on cellulolytic activity of the crystalline style extract. Substrate was CMC 70 medium; pH ; incubation time was two hours. The relationship between decreasing viscosity and increasing concentrations of reducing sugar during cellulolysis is presented in Figure 6. DISCUSSION There is general reluctance to attribute cellulase enzyme activity to higher ia because symbiotic microorganisms are i ivolved in cellulose breakdown in r animals. Indeed, Morton (1952. 1960), Newell (1953) and Barring- ton i have emphasi/ed the occurrence of spirochaetes in the crystalline style of many bivalves. Some, at least, of the cn/ymatic capability of the style is attrib- uted to these Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarenc
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