. Bulletin of the Department of Agriculture. Agriculture; Agriculture. EUROPEAN FOULBROOD. 13 Glucose gelatin plate.âAt rerrigeiulor teiuperulure aud within 3 days, tbe surface colonics l)egin to liquefy tln' gelatin, each liquefied area appearing somewhat as a minute drop of water. Agar slant.âIn one day numerous gray colonies cover the inoculated surface. Bouillon.âWithin a day the medium is uniformly and moderately clouded. Fermentation.âIn glucose, lactose, saccharose, levulose, maltose, and man- nite bouillons, a uniform clouding of the media occurs. The growth takes place in both ar
. Bulletin of the Department of Agriculture. Agriculture; Agriculture. EUROPEAN FOULBROOD. 13 Glucose gelatin plate.âAt rerrigeiulor teiuperulure aud within 3 days, tbe surface colonics l)egin to liquefy tln' gelatin, each liquefied area appearing somewhat as a minute drop of water. Agar slant.âIn one day numerous gray colonies cover the inoculated surface. Bouillon.âWithin a day the medium is uniformly and moderately clouded. Fermentation.âIn glucose, lactose, saccharose, levulose, maltose, and man- nite bouillons, a uniform clouding of the media occurs. The growth takes place in both arms of the tube, but is heavier in the open one. Considerable acidity, but no gas, is produced. Milk.âMilk is rapidly coagulated. Digestion of the coagulum follows. In from 3 to 5 days more than one-half has been changed. Within 24 hours the color is discharged in litmus milk, except at the top of the medium. In other respects it is like the plain milk. Potato.âNo visible growth. That growth in the potato water takes place is confirmed by microscopic examination. Gelatin stab.âLiquefaction along the line of puncture is appreciable after one day. In four days a cylinder of liquefied gelatin 1 cm. in diameter surrounds the original line of puncture and soon extends to the walls of the tube. I'athogencsis.âNo disease results when the brood of bees is fed cultures of Streptococoics apis either by the direct or indirect method. A rabbit and two guinea pigs inoculated with a pure cul- ture of Streptococcus apis were not sus- ceptible to infection with the species. BACTERIUM EUEYDICE The presence of this species in European foulbrood was pointed out by the writer in an earlier pubr lication (15). Among the second- ary invaders in larvae infected with Bacillus plwton, Bacterium eurij- ^'«- ^â -^<^oterium euryaice. dice is one of the earliest to be found. It is often present in consid- erable numbers. In plating for the species the stomach contents from larvae sick, but no
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