. Electronic apparatus for biological research . .^ - '/////yy/////A ... â|Hypodermic needle âCD ^ -Sliding surface Sliding surface Figure Section through a simple manipulator used for constructing a group of electrodes quantities of Picene or other wax can be applied with the aid of a simple tool resembling a soldering iron. This consists of a thin wire soldered to a piece of thick wire which is surrounded by a small low voltage heating coil. The wax is applied while the tips are supported in place, as otherwise they are drawn together by the surface tension of the wax when fluid. A com
. Electronic apparatus for biological research . .^ - '/////yy/////A ... â|Hypodermic needle âCD ^ -Sliding surface Sliding surface Figure Section through a simple manipulator used for constructing a group of electrodes quantities of Picene or other wax can be applied with the aid of a simple tool resembling a soldering iron. This consists of a thin wire soldered to a piece of thick wire which is surrounded by a small low voltage heating coil. The wax is applied while the tips are supported in place, as otherwise they are drawn together by the surface tension of the wax when fluid. A combination of longitudinal and lateral spacing has been used to form different patterns of recording electrodes. REFERENCES ^ HoDGKiN, A. L. and Huxley A. F. Resting and action potentials in single nerve fibres /. Physiol. 104 (1945) 176 ^ Curtis, H. J. and Cole, K. S. Membrane resting and action potentials from the squid giant axons J. Cell. Camp. Physiol. 19 (1942) 135 ^ Ling, G. and Gerard, R. W. The normal membrane potential of frog sartorius fibres J. Cell. Camp. Physiol. 34 (1949) 382 * Brixton, H. T, S. Hydrogen Ions London; Chapman & Hall. 1955 ^ Caldwell, P. C. Measurement of intracellular pH by means of a tungsten microelectrode /. Physiol. 124 (1954) p IP ® Eccles, J. C. The Physiology of Nerve Cells Oxford University Press. 1957 â ^ Nastuk, W. L. and Hodgkin, A. L. The electrical activity of single muscle fibres J. Cell. Comp. Physiol. 35 (1950) 39 8 MoREY, G. W. The Properties of Glass New York; Reinhold. 1938 ^ Hodgkin, A. L. Personal communication (1957) ^" Temtleton, C. C. Drawing of small uniform glass capillaries by electrical beating Rev. sci. Instriim. lA (1955) 880 ^^ Weale, R. a. a method for drawing microcapillary tubes /. Physiol. 112 (1950) 4P ^^ Brock L. G., Coombs, J. S. and Eccles, J. C. The recording of potentials from motor neurones with an intracellular electrode /. Physiol. 117 (1952) 431 ^^ Kao, , a method of making prefilled mi
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