. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 10-' 10-' 10-' 10-' 10- Concentration ETOH (molar) o _c a. o E <u LL B. 100- 90- 80- 70- 60- 50- 40- 30- 20- 10- 0- 123456 Exposure (days) Figure 1. Cumulative percent metamorphosis oflarvae ofPhestilla sihogae exposed to a series of ethanol (ETOH) solutions for 6 days. Larvae were 11 days old at the beginning of experiments; plotted results are pooled means and S. D.'s of 6 experiments, each with 2 replicate assay wells per ETOH concentration, each well containing ca. 20 lar- vae. (A) Metamorphic response plotted as
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 10-' 10-' 10-' 10-' 10- Concentration ETOH (molar) o _c a. o E <u LL B. 100- 90- 80- 70- 60- 50- 40- 30- 20- 10- 0- 123456 Exposure (days) Figure 1. Cumulative percent metamorphosis oflarvae ofPhestilla sihogae exposed to a series of ethanol (ETOH) solutions for 6 days. Larvae were 11 days old at the beginning of experiments; plotted results are pooled means and S. D.'s of 6 experiments, each with 2 replicate assay wells per ETOH concentration, each well containing ca. 20 lar- vae. (A) Metamorphic response plotted as a function of ETOH concen- tration on days 2, 4 and 6; larvae were moribund and most eventually died at concentrations > A/ (4%). (B) Response as a function of duration exposure to ETOH at , , and M; maximal larval response was assayed with a coral chip, and "spontaneous" metamor- phosis was monitored in wells containing larvae and seawater alone. FSW, and two contained M ethanol and FSW. The final pair of dishes contained FSW alone. The larvae were scored for metamorphosis on each of the next 3 days; animals were determined metamorphosed when their vela had completely disappeared. Results Assays for metamorphic response to solvents Ethanol and methanol. Eleven-day-old veligersof/Vu'- stilla sibogae metamorphosed when exposed to both eth- anol (Fig. 1A-B) and methanol (Fig. 2A-B). Very few of the larvae (<5%) metamorphosed over the 6 days of the experiments in filtered seawater alone, and 85-100% of the larvae metamorphosed within 1-2 days of exposure to a living chip of the coral Porites compressa (Figs. IB, 2B). Maximal response to ethanol was between these control values, with 60-80% oflarvae metamorphosing in M ethanol (Fig. 1A). At least a few larvae meta- morphosed at all doses between 5 X 10~4 and A/, but larvae were always moribund and usually died in solu- tions at and above M (ca. 4%) ethanol. Maximal response to met
Size: 1789px × 1396px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
