. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 10 12 14 16 18 20 22 Length of Shell (cm) Figure 1. Pheophytin a per gram of siphonal mantle (wet weight) as a function of shell length in Tridacna maxima (filled symbols) and 7". squamosa (open symbols). The line and equation are results of linear, least-squares regression, all data taken together. Two data points for 17-cm clams are shifted laterally for clarity. absolute methanol to place concentrations on the linear parts of calibration curves. In Pohnpei. tissue samples were prepared, weighed, and inserted in vials
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 10 12 14 16 18 20 22 Length of Shell (cm) Figure 1. Pheophytin a per gram of siphonal mantle (wet weight) as a function of shell length in Tridacna maxima (filled symbols) and 7". squamosa (open symbols). The line and equation are results of linear, least-squares regression, all data taken together. Two data points for 17-cm clams are shifted laterally for clarity. absolute methanol to place concentrations on the linear parts of calibration curves. In Pohnpei. tissue samples were prepared, weighed, and inserted in vials as solid blocks of tissue to minimize po- tential loss of DMS. To assure that extractions of DMSP, DMS, and chlorophyll from samples into methanol were complete, tissue samples were removed briefly from sample vials after completion of the measurements described above and minced with scissors into small pieces (ca. 1 mm greatest thickness) that fell back into the vials (the proce- dure required about 1 min per sample). After 24-48 h, all measurements were repeated. Chlorophyll concentrations were not altered by mincing, and concentrations of pooled DMSP and DMS (measured as earlier described) were al- tered little, if at all (possibly 3%-5% in the case of mantle samples). The mincing test demonstrated that extraction from whole tissue was complete or virtually complete, and all assays for a sample were averaged to obtain the results reported. A second check on our technique was to test whether the high tissue DMSP concentrations we encoun- tered might be so high as to saturate the methanol. The highest concentration of DMSP in the methanol in any tissue-sample vial was mA/. Without attempting to de- termine the absolute solubility of DMSP in methanol, we ascertained that pure DMSP sufficient to make a solution three times as concentrated dissolved rapidly in methanol. Thus, saturation of the methanol in the sample vials did not occur. Total amounts of pooled DMSP and DMS (
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