. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 20 S. D. PAINTER ET Positive Negative No Choice Nonlaycr Konlayer Nonlayer No Animal A brasiliana A. californica Native Att RccombAn Recomb Alt Recomb AR RecombAn Material Added Figure 3. Both native and recombinant attractin are attractive; attractin acts in conjunction with other odors; and the animal-derived factor is not species-specific. The number of Aplysia brasiliana individuals attracted to a non-laying conspecific (Nonlayer) was increased by placing I pmol of either native attractin (Nonlayer Native Att) or re
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 20 S. D. PAINTER ET Positive Negative No Choice Nonlaycr Konlayer Nonlayer No Animal A brasiliana A. californica Native Att RccombAn Recomb Alt Recomb AR RecombAn Material Added Figure 3. Both native and recombinant attractin are attractive; attractin acts in conjunction with other odors; and the animal-derived factor is not species-specific. The number of Aplysia brasiliana individuals attracted to a non-laying conspecific (Nonlayer) was increased by placing I pmol of either native attractin (Nonlayer Native Att) or recombinant attractin (Nonlayer Recomb Att) in the adjacent seawater. In each assay, animals chose between a stimulus in one arm and no stimulus in the other. Fewer A. brasiliana individuals were attracted to recombinant attractin when the stimulus did not contain a non-laying conspecific (No Animal Recomb Att; 1 pmol). About the same number of A. brasiliana individuals were attracted to the specimen of A. californica with recombinant attractin (A. californica Recomb Att; 1 pmol) as were attracted to the specimen of A. brasiliana with recombinant attractin (A. brasiliana Recomb Att; 1 pmol). Animals do not release attractin unless they are laying eggs; therefore, the combined odor of a non-laying animal and attractin produces a qualitatively different stimulus from attractin alone. The data confirm that attractin func- tions as part of a bouquet of scents and led us to ask. Does the animal-derived pheromone have to come from a con- specific or can it come from a different species of Aplysia, perhaps accounting for the presence of multiple species at an aggregation? This would also be consistent with reports of multiple species showing up at one aggregation in the field. The results of experiments examining whether the stim- ulus animal needs to be a conspecific in order for attractin to be attractive are shown in Figure 3. When 1 pmol of recombinant attractin was placed in the se
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology