Edible and poisonous mushrooms of Edible and poisonous mushrooms of Canada ediblepoisonousm00grov Year: 1979 EDIBLE AND POISONOUS MUSHROOMS OF CANADA pileus on a piece of white paper with the lamellae down, wrapping it carefully in waxed paper, and laying it flat on the bottom of the basket. A good spore deposit may be obtained by the time one returns from the collecting trip. If the spore deposit is white to yellow, the amyloid reaction of the spores should be determined. This reaction is determined by placing some of the spores on a glass slide and adding a drop of a solution (Meizer's Reag
Edible and poisonous mushrooms of Edible and poisonous mushrooms of Canada ediblepoisonousm00grov Year: 1979 EDIBLE AND POISONOUS MUSHROOMS OF CANADA pileus on a piece of white paper with the lamellae down, wrapping it carefully in waxed paper, and laying it flat on the bottom of the basket. A good spore deposit may be obtained by the time one returns from the collecting trip. If the spore deposit is white to yellow, the amyloid reaction of the spores should be determined. This reaction is determined by placing some of the spores on a glass slide and adding a drop of a solution (Meizer's Reagent) made up of grams potassium iodide, grams iodine, 20 grams distilled water and 20 grams chloral hydrate. If the spores turn gray-blue to blackish blue they are amyloid and if there is no reaction they are nonamyloid. The reaction can be observed with the microscope if one is available, or, if not, it can be seen by simply holding the sHde over white paper. The reaction should be noted within a few minutes of making the test and it is more reliable if tested on spores that have first been dried. A few species, particularly in Lepiota, will give a falsely amyloid or pseudoamyloid reaction and the color will be reddish brown. This reaction is of considerable importance in taxonomic work. For example, the genus Leucopaxillus contains some species that were formerly in Clitocybe and some that were in Tricholoma. These are beheved to form a natural group and can be recognized by the amyloid reaction of the spores. In '0$m!^iii Figure 45. Spore print of a mushroom. 16
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