. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HEMOGLOBIN EVOLUTION 229 RESULTS Instead of presenting all data in the form of the usual "oxygen dissociation curve," the linear transformation based on the Hill approximation, y = 100 + is used in Figures 1 and 3 (Lemberg and Legge, 1949). The variables y and p are the per cent oxyhemoglobin and the partial pressure of oxygen, respectively. 100-y Hemoglobin Solutions • o o « e o • ( » 2thr«. later) « * » Erythrocyte Suspensions pH * 10 100 FIGURE 1. Oxygen e
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HEMOGLOBIN EVOLUTION 229 RESULTS Instead of presenting all data in the form of the usual "oxygen dissociation curve," the linear transformation based on the Hill approximation, y = 100 + is used in Figures 1 and 3 (Lemberg and Legge, 1949). The variables y and p are the per cent oxyhemoglobin and the partial pressure of oxygen, respectively. 100-y Hemoglobin Solutions • o o « e o • ( » 2thr«. later) « * » Erythrocyte Suspensions pH * 10 100 FIGURE 1. Oxygen equilibrium of hemoglobin of the California hagfish, Polistotrcma stouti. Three to four per cent hemoglobin solutions in potassium phosphate buffer; final ionic strength = Erythrocyte suspensions in isotonic phosphate-buffered saline with Karo added as explained in text. Temperature = 18° C. The solid lines are drawn arbitrarily with a slope (n) = and a />.,„ corresponding to approximately physiological pH's. That value of p for which y equals 50% is the />.|0. The "sigmoid coefficient," n, is a measure of the heme-heme interactions. Hence, />-„ is an inverse measure of the oxygen affinity, and n determines the shape of the oxygen dissociation curve. If the slope of the transformation, log [v/(100 -- y)] as a function of log p, is one, then the hemes are totally independent—, there is no heme-heme interaction. As can be seen from Figures 1 and 3, where the solid lines are drawn with a slope of , this is true of hagfish hemoglobin inside and outside the erythrocyte, and at high and low Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual rep
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