. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . tory, float the cover-slip off with saltsolution, dry, and mount in balsam. Under no conditionsshould plain water come in contact with this preparationat any stage; otherwise no capsules will appear. Second method (Welchs method): a) Prepare a very thin film in the usual manner froma 24-hour-old milk-culture. h) Cover with glacial acetic acid for 5 seconds. c) Wash acid off with carbol-fuchsin. d) Wash stain off with a per cent, sodiumchlorid solution. c) Examine in salt solution. /) If satisfacto
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . tory, float the cover-slip off with saltsolution, dry, and mount in balsam. Under no conditionsshould plain water come in contact with this preparationat any stage; otherwise no capsules will appear. Second method (Welchs method): a) Prepare a very thin film in the usual manner froma 24-hour-old milk-culture. h) Cover with glacial acetic acid for 5 seconds. c) Wash acid off with carbol-fuchsin. d) Wash stain off with a per cent, sodiumchlorid solution. c) Examine in salt solution. /) If satisfactory, mount in balsam. Plain water should not come in contact with thispreparation at any stage. The capsule should be ob-served as a lightly stained zone with well-defined outlinearound the deeply stained organism. 78 LABORATORY GUIDE IN BACTERIOLOGY 3. Special study B.—Inoculation of mouse with a) Fasten the mouse in the holder (Fig. 32). b) Shave a place on the back inunediately above thetail. c) Wash with a solution of mercuric chlorid (i: 1000),followed by d) Inject of a milk-culture of ilicr. lanceo-latus. e) When dead, perfonn an autopsy, and study thelesions in the usual manner. /) Make cultures in milk and on slant-agar fromthe hearts blood or the spleen. g) Make a capsule stain from the hearts blood,spleen, or other organs. CHAPTER XIII THE INTESTINAL GROUP This chapter is devoted to the study of the intestinalgroup of organisms. This collective group mayconveniently be subdivided into four subgroups: Subgroup I: the colon group.—This group includesdifferent varieties of Bacillus coli, B. lactis aerogenes, andsome species of B. acidi lactici. Subgroup 2: the hog-cholera, B. enteriiidis, or inter-mediate group.—This group includes B. cholerae suis, (several varieties), B. enteritidis, and The term intermediate is assigned to thisgroup, because it resembles the colon group on the onehand, and the typho
Size: 1978px × 1264px
Photo credit: © Reading Room 2020 / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookdecade1900, bookpublisherchicagotheuniversi
