. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. A B C D FIGURE 1. Camera lucida drawings of the anterior half of a living explant during its develop- ment on a medium containing Ringer, l(h2M glucose, and 5 X KHJl/ sodium fluoride. A. Stage explanted; B. 8 hours later; C. 18 hours after explanation; D. 38 hours after explantation. Note the degenerating heart mesoderm. sufficient amount (2 X 10~2M) of pyruvate for glucose (Table III) suggests that the inhibitor is blocking enolase activity. If this is true, the greater sensitivity of the heart as compared with the developi
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. A B C D FIGURE 1. Camera lucida drawings of the anterior half of a living explant during its develop- ment on a medium containing Ringer, l(h2M glucose, and 5 X KHJl/ sodium fluoride. A. Stage explanted; B. 8 hours later; C. 18 hours after explanation; D. 38 hours after explantation. Note the degenerating heart mesoderm. sufficient amount (2 X 10~2M) of pyruvate for glucose (Table III) suggests that the inhibitor is blocking enolase activity. If this is true, the greater sensitivity of the heart as compared with the developing brain would indicate its greater de- pendence upon a glycolytic energy source. Since the effect of fluoride on respira- tion is relatively indirect and slight (see Gemmill. 1939, for discussion of the mechanism of fluoride inhibition) compared with the effect of iodoacetate, one would expect that its interference with the metabolic basis of brain-formation would be correspondingly less drastic. Eventually, the piling up of metabolic intermediates in the presence of fluoride would presumably interfere with the glycoly- tic and oxidative pathways above pyruvate. Indeed, fluoride does have a delayed inhibitory effect upon the nervous system during the second day of cultivation (Fig. 1). 5 It is generally held that fluoride inhibits the enzyme enolase which catalyzes the glycolytic reaction: 2-Phosphoglyceric acid ^ Phosphoenolpyruvic acid, by formation of a magesium- fluoride phosphate complex (Meyerhof and Kiessling, 1935; Warburg and Christian, 1942).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred C
Size: 1244px × 2010px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
