. Bacteria in relation to plant diseases. Bacteria; Plant diseases. WILT-DISEASES OF TOBACCO. 239 The following statements are taken from the more recent paper by Uyeda (1905): The disease is widespread in Japan and has been known for at least 25 years, having been de- scribed in 1S81 in Ensoroku, a book on tobacco culture. Since that time it has been written upon with special reference to methods of prevention by T. Kugahara in the experiment station of Huku- shima-Ken (1891) and at about the same time by Dr. S. Hori, in the Imperial Central Agricultural Experiment Station at Tokyo. Prof. Y.
. Bacteria in relation to plant diseases. Bacteria; Plant diseases. WILT-DISEASES OF TOBACCO. 239 The following statements are taken from the more recent paper by Uyeda (1905): The disease is widespread in Japan and has been known for at least 25 years, having been de- scribed in 1S81 in Ensoroku, a book on tobacco culture. Since that time it has been written upon with special reference to methods of prevention by T. Kugahara in the experiment station of Huku- shima-Ken (1891) and at about the same time by Dr. S. Hori, in the Imperial Central Agricultural Experiment Station at Tokyo. Prof. Y. Kozai, director of the experiment station at Nishigahara, has also isolated a schizomycete, which he holds to be the cause of the disease. This is the same as that described by Uyeda, who did his work at that station. The disease is variously known to the Japanese as "stem-rot," "black-leg,'' and "; Uyeda's studies were begun in 1899 and continued nearly 5 years. The disease is widespread in the province of Hitachi, and occurs also in the province of Sagami and Fig. 126.* The signs of the disease are much like those of the Granville wilt. Compare fig. 126 with pi. 33, and pi. 41, figs. 1, 2, 7, with pi. 42, for brown stripes on the green stems. The disease occurs from June to September on plants of all ages. The first sign is the sudden wilt of the foliage, after which the leaves become yellow. The stem then becomes black and the roots decay. When the juice from diseased plants or a pure culture of the bacillus is placed on healthy tobacco leaves, they begin to blacken and to show brown spots within one or two weeks. The principal veins of the leaf are then hollowed out and destroyed. The infection experiments were made on young and old plants at Nishigahara. These are not described. Old leaves sometimes show wavy black spots bordering the veins, where the bacteria are said to have entered by the stomata. The organism often enters
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