Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . Fig. 26.—Liboriuss method. Fig. 27.—Hesses method of makinganaerobic cultures (McFarland). by means of a long platinum rod with small loop, the move-ment being a rotary vertical one, and the rod going to thebottom of the tube. The gelatin is next quickly solidified under ice; very littleair is present. The anaerobic germs wiU grow from the CULTIVATION OF ANAEROBIC BACTERIA 83 bottom upward, and any aerobins present will develop firston top, this method being one of isol
Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . Fig. 26.—Liboriuss method. Fig. 27.—Hesses method of makinganaerobic cultures (McFarland). by means of a long platinum rod with small loop, the move-ment being a rotary vertical one, and the rod going to thebottom of the tube. The gelatin is next quickly solidified under ice; very littleair is present. The anaerobic germs wiU grow from the CULTIVATION OF ANAEROBIC BACTERIA 83 bottom upward, and any aerobins present will develop firston top, this method being one of isolation. From the anaerobic germ grown in the lower part a stabculture is made into another tube containing three-quartersgelatin, the material being obtained by breaking test-tubewith the culture. (See Fig. 26.) Hesses Method.—A stab-culture having been made with. Fig. 28.—Frankels method ofmaking anaerobic cultmres (McFar-land). Fig. 29.—Buchners method ofmaking anaerobic cultures (Mc-Farland). anaerobic germs, gelatin in a semisolid condition is pouredinto the tube until it is full, thus displacing the air (Fig. 27).Esmarchs Method.—^Having inoculated a tube, the gela-tin is rolled out on the walls of the tube, a roU culture,and the rest of the interior is filled with gelatin, the tubebeing held in ice-water. The colonies develop upon the sidesof the tube and can be examined microscopically. 84 ESSENTIALS OF BACTERIOLOGY Gases like Hydrogen to Replace the Oxygen.—Severalarrangements for passing a stream of hydrogen through theculture:Frankel puts in the test-tube a rubber cork containing twoglass tubes, one reaching to the bot-tom and connected with a hydrogenapparatus, the other very short,both bent at right angles. Whenthe hydrogen has passed throughfrom ten to thirty minutes, theshort tube is annealed and then theone in connection
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