. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 254 READ ET FIGURE 9. Mastigotes. A-G The number of undulipodia per cell vary from 1 to as many as 11. Bar = 5 j/m. Growth and reproduction The new strain off. jugosus grew extremely rapidly. New isolates from the field entirely covered the B bacillus colonies with cysts within 3 days at room temperature (color plate I-IV). About sixty cysts per colony developed from plated colonies using a loop. After about a year in culture the growth rate slowed somewhat: it took from 4 to 5 days to entirely replace the food colonies
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 254 READ ET FIGURE 9. Mastigotes. A-G The number of undulipodia per cell vary from 1 to as many as 11. Bar = 5 j/m. Growth and reproduction The new strain off. jugosus grew extremely rapidly. New isolates from the field entirely covered the B bacillus colonies with cysts within 3 days at room temperature (color plate I-IV). About sixty cysts per colony developed from plated colonies using a loop. After about a year in culture the growth rate slowed somewhat: it took from 4 to 5 days to entirely replace the food colonies with cysts. The ATCC P. jugosus grew more slowly, not forming visible cysts at all until after the 12th day. It never formed populations as dense as the Baja California isolate on any media tested (for example the maximum number of cysts per colony was about 30 in the same test that the Baja California strain developed about 60 cysts per colony). Even after transfer from higher salt concentration ( M NaCl) the ATCC P. jugosus grew very slowly and to low cell densities on our routine MnAc media (Fig. 16). We interpret the rounded forms to be active feeding stages. In young cultures virtually devoid of monopodial amoebae the effects of P. jugosus on bacteria were easily seen. Motility was lost and the bacteria became severely clumped and elon- gated. Apparently P. jugosus arrested bacterial cell division, for when infected with P. jugosus the bacilli would grow to up to 10 times their normal length and in some cases spaghetti-like masses of unhealthy-appearing bacteria were seen (color plate V, Fig. 10A, compare with Fig. 3). Thread-like material in which bacteria were embedded could be seen in scanning electron micrographs (Fig. 10B). The material,. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine B
Size: 995px × 2512px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
