. Bonner zoologische Beiträge : Herausgeber: Zoologisches Forschungsinstitut und Museum Alexander Koenig, Bonn. Biology; Zoology. Heft 3/4 19/1968 The Relationships of the Seed-snipe 241 frozen, and stored at —75° C. Prior to electrophoresis a small sample was thawed and diluted with starch-gel buffer. Starch-gel electrophoresis (Smithies, 1955, 1959a, b) with a discontinuous buffer system (Poulik, 1957), modified according to Sibley, Corbin, and Haavie (in press), was used. The starch buffer was composed of M Tris(hydroxymethyl)aminomethane, M citric acid, M lithium hydroxid
. Bonner zoologische Beiträge : Herausgeber: Zoologisches Forschungsinstitut und Museum Alexander Koenig, Bonn. Biology; Zoology. Heft 3/4 19/1968 The Relationships of the Seed-snipe 241 frozen, and stored at —75° C. Prior to electrophoresis a small sample was thawed and diluted with starch-gel buffer. Starch-gel electrophoresis (Smithies, 1955, 1959a, b) with a discontinuous buffer system (Poulik, 1957), modified according to Sibley, Corbin, and Haavie (in press), was used. The starch buffer was composed of M Tris(hydroxymethyl)aminomethane, M citric acid, M lithium hydroxide, and M boric acid at pH The bridge buffer consisted of M lithium hydroxide and M boric acid, pH Electrophoresis was effected by a constant current of 35 ma (hence voltage varied from 400—600 V) until a bromphenol blue dye marker moved 8 cm from the application slot (about 4—5 hours). The gel was removed, sliced, and stained for total protein with amido black 10 B. Gels were destained by successive washings with °/o (v/v) acetic acid and photographed. Results The interpretation of electrophoretic patterns is discussed elsewhere (Sibley, in press; Sibley and Ahlquist, in press). The principal components of an egg white pattern of a non-passerine bird are diagrammed in Figure 1. Figure 2 shows the egg white patterns Lysozyme Component 18 Globulins Ovalbumin. Figure 1. Diagram of the starch-gel electrophoretic pattern of the egg white proteins of a non-passerine bird. The ( + ) indicates the anodal direction; the (—) the cathodal direction. of Thinocorus orbignyianus and Attagis gay i along with 13 other genera representing 11 of the families recognized by Wetmore (1960). The patterns of Thinocorus and Attagis have ovalbumins of identical mobilities and differ but slightly in the mobilities of the ovomucoids. Attagis has two of its three. Please note that these images are extracted from scanned page images that may have been digitally en
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