. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. NUTRIENT MEDIA -AND METHODS OF CULTIVATION. 131. process very little air re-enters the jelly, and colonies of even strictly anaerobic bacteria will develop in the lower part of the tube. The drawback is the difficulty encountered in examining the colonies, and in preparing sub-cultures. For this purpose the tube must be broken, or carefully warmed until the jelly can be shaken out. Esmarch first prepares a roll culture,
. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. NUTRIENT MEDIA -AND METHODS OF CULTIVATION. 131. process very little air re-enters the jelly, and colonies of even strictly anaerobic bacteria will develop in the lower part of the tube. The drawback is the difficulty encountered in examining the colonies, and in preparing sub-cultures. For this purpose the tube must be broken, or carefully warmed until the jelly can be shaken out. Esmarch first prepares a roll culture, and when the gelatine film, has set, the tube is completely filled with liquefied gelatine which has been cooled down almost to the temperature at which it solidifies. The same diflficulty arises £ in the previous method, in the examination of the colonies. Buchner places the culture tube inside a much larger tube containing a small quantity of pyro- gallic acid and closed with a gutta-percha cap. The pyrogallic acid absorbs the oxygen, but the method is not altogether success- ful. The most satisfactory plan is to exhavist the air with an air pump, or to substitute an atmosphere of hydrogen which does not affect the growth of the bacteria. "Various forms of flasks and tubes for cultivating ^^^ 's Anaekoeio Tube-culture" bacteria have been devised, jj_„^ gjass tube through which hydrogen is which can be easily con- passed; b, exit tube; e, india-rubber nected with an exhausting ^^X^LANnr "*""''" "^'^ '""*'' apparatus, and • readily sealed by the flame of the blowpipe when the air has been removed. If hydrogen is employed the most convenient plan is to use a Zipp's apparatus, from which the hydrogen is passed through two bottles, one containing a solution of lead, to remove any sulphuretted hydrogen, and the other pyrogallic acid, to intercept any Please note that these images are extracted from scanned page
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