. Pathological technique; a practical manual for workers in pathological histology and bacteriology. g position in such away as to give a good view to the operator of the surface ofthe media in each, while the cotton stoppers are removedand held between the fingers of the same hand (Fig. 19).The object of holding the tubes in a slanting position is tooffer less chance of contamination from bacteria gaining en-trance to the culture-medium from the air. The platinum wire, which is manipulated by the righthand, is first sterilized by holding in the Bunsen flame untilit glows, and then cooled by c
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. g position in such away as to give a good view to the operator of the surface ofthe media in each, while the cotton stoppers are removedand held between the fingers of the same hand (Fig. 19).The object of holding the tubes in a slanting position is tooffer less chance of contamination from bacteria gaining en-trance to the culture-medium from the air. The platinum wire, which is manipulated by the righthand, is first sterilized by holding in the Bunsen flame untilit glows, and then cooled by contact with the media to beinfected, after which its free end is carefully brought incontact with the discrete colony or pure culture-growth,and immediately inserted into the sterile tube to inoculateit. The manner of inoculating the sterile culture-mediumin the other tube with the infected platinum wire will varywith the form and character of the culture desired. If the medium to be inoculated is a fluid one, the wire issimply immersed in it and moved back and forth once or CULTURE METHODS. 217. twice. If the medium be a solid one in the form of a slant,the infected end of the wire is drawn over the surface onceor twice from the bottom of the slant to its upper end; orif the solid medium in the tube be arranged for a stab cul-ture (seepage 196), the infected wire is to be plunged oncethrough the center of themass to the bottom of thetube. After the tubes havebeen inoculated as above in-dicated, the wire is to be im-mediately withdrawn andthe cotton stoppers are then to be placedin the incubator for devel-opment. Gelatin cultures,however, must not be sotreated, but are to be kept atroom-temperature, for theheat of the incubator wouldcause the gelatin to becomefluid. These details as to the manner of manipulating the cul-ture-tubes, cotton stoppers, and platinum wire also apply tothe procedure described below. Method of Isolation of a Bacterium in Pure Cul-ture fro
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