. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. G3IUD] 8 S. • • •*• • .» .** - . • a < a a 0500 A A A A 5 g 5 5 Figure 1. (A) Typing at microsatellite locus 811 of genotype combinations A and B (divided into subclones AI-A3 and BI-B4), Allelic typing (21) of the partners is shown in brackets. Compartments sampled are the hcmolymph (H); a tissue fragment (T); a single zooid (Z) and if present, from the gonads (G) of the same zooid. Chimeras were first sampled 25 days after vascular anastomosis, after subcloning (numbers in parentheses denote days after subclonin
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. G3IUD] 8 S. • • •*• • .» .** - . • a < a a 0500 A A A A 5 g 5 5 Figure 1. (A) Typing at microsatellite locus 811 of genotype combinations A and B (divided into subclones AI-A3 and BI-B4), Allelic typing (21) of the partners is shown in brackets. Compartments sampled are the hcmolymph (H); a tissue fragment (T); a single zooid (Z) and if present, from the gonads (G) of the same zooid. Chimeras were first sampled 25 days after vascular anastomosis, after subcloning (numbers in parentheses denote days after subcloning). and upon sexual maturation. Predetermined alleles in the population (A = 216 bp, B = 232. C = 236. D = 240. E = 268) are depicted at the left side. Horizontal bar-lines group simultaneous sampling of subclones, or samples from several compartments of a single subclone. An overnight exposure of the film is shown. (B) A pictorial outline of genotype combination G depicting the corresponding subclones. The chimera was cut into 4 subclones. 20 days after fusion (marked lines in the figure): Gl. the original chimera with three systems; G2. a single system, an outgrowth from genotype BE; G3 and G4, each a single system originating from genotype DD. Thirty days later (50 days after chimera formation) subclone Gl disconnected naturally along the region of fusion, into subclone Gl [BE] and subclone Gl [DD]. The bar line represents I mm. (O Typing at microsatellite locus 81 1 of genotype combinations G, H. J. K. L. Allelic typing of the partners in each chimera is shown in brackets. Sampling days and compartments as in Figure la. Subclone sampling from genotype combination G is as depicted in Figure Ib. Genotype com- binations H. J, K, L were sampled after the resorption of one partner in each (numbers are for day after fusion; numbers in parentheses denote days after resorption). An overnight exposure of the film is shown. (D) Typing of three recipient colonies which we
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
