ANTIMICROB. SUSCEPTIBILITY TEST
Photo essay from laboratory. Hospital of Meaux (77), France. Laboratory of bacteriology. Antimicrobial susceptibility test with Etest. The antimicrobial susceptibility test enables to determine the sensibility to different antibiotics of the bacterium infecting the patient. To measure quantitatively this sensibility, it is possible to use strips impregnated of a growing gradient of an antibiotic (Etest). Thefore, the bacterial culture is seeded on a petri dish and one of various strips of antibiotic are placed there. During the incubation, an inhibition ellipse of the bacterial growth forms around the strip. The value of the minimum inhibitory concentration (MIC, weakest value of antibiotic inhibiting the visible growth) is read on the strip, at the intersection of the inhibition area and the strip. This method enables to determine which antibiotic will be the most efficient to treat the patient. Here, test de la sensibility of the bacterium to different beta-lactams (AML : amoxicillin, CT : cefotaxime, PG : penicillin G).
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Photo credit: © B. BOISSONNET / BSIP / Alamy / Afripics
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