. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 224 k \\K M. l-MSIIKK, JR. authors. Chromatograms were also examined under ultraviolet light, and many of the aryl-ngKcone moieties of the glucosides were visualized. Protein fractions were examined by disc electrophoresis ( Reisfeld ct al., 1962) on acrylamide -el incorporating Tris : glycine buffer, pi 1 with a potential of J(K) yolts. The protein bands were localized with amido black. RESULTS Total Animal Preparation Hydrolysis of carbohydrates Assay of the supernatant of total animal homogenates ( Fraction II) afford
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 224 k \\K M. l-MSIIKK, JR. authors. Chromatograms were also examined under ultraviolet light, and many of the aryl-ngKcone moieties of the glucosides were visualized. Protein fractions were examined by disc electrophoresis ( Reisfeld ct al., 1962) on acrylamide -el incorporating Tris : glycine buffer, pi 1 with a potential of J(K) yolts. The protein bands were localized with amido black. RESULTS Total Animal Preparation Hydrolysis of carbohydrates Assay of the supernatant of total animal homogenates ( Fraction II) affords some idea of the number of carbohydrases present in Blabcrns craniifcr (Table I ). In addition to those substrates shown, glycogen and starch were hvdrolyzed ; however, cellulose and methyl cellulose were not hydrolyzed. Precipitation of Fraction II with ethanol at -7° C. partially separated and increased the specific- activity of the preparations (Table II). The a-glucosidase active against maltose precipitated largely in the 70%: ethanol fraction, while the /?-glucosidase activity precipitated out in the 50% and 60^0 fractions. Trehalase was also equally distributed in the 50% and 60% 0 40 60 80 MINUTES 100 120 140 KltVct ot' pi I and iiuTi-asinn tiim- on rn/ymatic activity. IMilhalaU- (N'a1) hul'- -^.0, pi I , pH , O-O pH Conditions: 10 Amok's c iflfer, ml. enzyme (40', to 60% ethanol fraction, whole- animal).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
