. The development of a cosmid map of chromosome 12p13. Sweet Briar College; Chromosomes. of kilobases. There were eight cosmids on the dot blot that demonstrated strong labeling reactions when exposed to the labeled probe during hybridization (FIGURE23). The positive cosmids were c 1C6. c69B7. c88A9, c 167H8. c 179F11. c 189G12. c 192A8. and c200B8. Cosmid 128D6 was a positive control, as determined by PCR mapping methods, but did not label in this FiciURi 23. Hybridization ofdigoxigenin labeled DI2S19I4 STS marker to cosmid dot blot. Eight cosmids labeled most strongly i
. The development of a cosmid map of chromosome 12p13. Sweet Briar College; Chromosomes. of kilobases. There were eight cosmids on the dot blot that demonstrated strong labeling reactions when exposed to the labeled probe during hybridization (FIGURE23). The positive cosmids were c 1C6. c69B7. c88A9, c 167H8. c 179F11. c 189G12. c 192A8. and c200B8. Cosmid 128D6 was a positive control, as determined by PCR mapping methods, but did not label in this FiciURi 23. Hybridization ofdigoxigenin labeled DI2S19I4 STS marker to cosmid dot blot. Eight cosmids labeled most strongly in this procedure. These were clC6, c69B7, c88A9, CI67H8. , CI92A8. andc200B8. 11 Rixiim TioN Enzymi: Dices I ions. Restriction endonucleases are often used to fragment DNA for studying the ivlationships between several dilTerent pieces of DNA of interest. Restriction enzyme digestions were used here to map the relationships between cosmids that were positive for particular STS markers. Three sets of cosmids were tested using restriction 39. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Belk, Stephanie Heather.
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Keywords: ., bookcentury1900, booksubjectchromo, booksubjectsweetbriarcollege
