. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 710 Tuberculosis lung for the purpose. The tissues are first cooked in a steam ster- ilizer, then cut into prisms, placed in a Roux tube, an addition of 6 to 8 per cent, glycerin-water added, so as to bathe the lower part "of the tissue and keep it moist, and the whole then sterilized in the autoclave. The organisms are planted upon the tis- sue, the top of the tube closed with a rubber cap, and the culture placed in the thermostat. The tubercle bac
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 710 Tuberculosis lung for the purpose. The tissues are first cooked in a steam ster- ilizer, then cut into prisms, placed in a Roux tube, an addition of 6 to 8 per cent, glycerin-water added, so as to bathe the lower part "of the tissue and keep it moist, and the whole then sterilized in the autoclave. The organisms are planted upon the tis- sue, the top of the tube closed with a rubber cap, and the culture placed in the thermostat. The tubercle bacilli -grow quickly and luxuriantly. Bouillon.—Upon bouillon to which 6 per cent, of glycerin has been added the bacillus grows well, provided the trans- planted material be in a condition to float. The organism being purely aerobic grows only at the surface, where a much wrinkled, creamy white, brittle pellicle forms. Non-albuminous —Instead of re- quiring the most concentrated albuminous media, as was once supposed, Proskauer and Beck* have shown that the organism can be made to grow in non-albuminous media containing asparagin, and that it can even be induced to grow upon a mix- ture oi commercial ammonium carbonate, per cent.; primary potassium phos- phate, per cent.; magnesium sulphate, per cent.; glycerin, per cent. Tuberculin was produced in this mixture. Gelatin.—The tubercle bacillus can be grown in gelatin to which glycerin has been added, but as its development takes place only at 37° to 38°C., a temperature at which gelatin is always liquid, its use for the purpose has no advantages. Appearance of the Cultures.—Irre- spective of the media upbn which they are grown, cultures of the tubercle bacillus present certain characteristics which serve to separate them from the majority of other organisms, though insufi&cient to enable one to identify them with certainty. The bacterial masses make their ap- As a rule very little growth ca
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1919
