. Contributions from the Botanical Laboratory, vol. 12. Botany; Botany. 6i8 AMERICAN JOURNAL OF BOTANY [Vol. 22, (iq^2). Cyclohexanol would not wet most latex particles upon which it was tried, but caused them to swell to approximately ten times their size before the interface overtook them. This phenomenon was probably due to absorption of cyclohexanol dissolved in the water Once swollen the particles were not wet by the oil but were carried ahead ^"definitely Th s oil was therefore unsatisfactory. However, sperm oil and triolein (Kahl- baum) were found to yield good results. The sample
. Contributions from the Botanical Laboratory, vol. 12. Botany; Botany. 6i8 AMERICAN JOURNAL OF BOTANY [Vol. 22, (iq^2). Cyclohexanol would not wet most latex particles upon which it was tried, but caused them to swell to approximately ten times their size before the interface overtook them. This phenomenon was probably due to absorption of cyclohexanol dissolved in the water Once swollen the particles were not wet by the oil but were carried ahead ^"definitely Th s oil was therefore unsatisfactory. However, sperm oil and triolein (Kahl- baum) were found to yield good results. The sample of sperm oil used was brown in color and appeared to be fairly viscous. Although results obtained with it checked closely with those noted with triolein, its source unfortu- nately could not be ascertained. The intensities of flocculation of the diluted latices in distilled water were noted immediately after tapping and again after 24 hours. The results are shown in table i. Intensities of flocculation are indicated as follows. O, clear; ±, slightly opaque; +, small clots; + + , clots m suspension; + + +, flocculated in part; + + + + , flocculated completely. It is possible that flocculation in distilled water indicates a globulin or other water- insoluble protein surface (Gortner, 1929); such dumped particles usually adhere to the microscope slide. The ninhydrin and xanthoproteic reactK^ns were also used as rough indicators of proteins. It is probable that these tests are obscured to some extent by non-protein constituents of the Of the two reactions, the ninhydrin test is believed to be the more reliable. Intensities are indicated in table i in the following manner: o, no reaction; + , very weak; ++. pale color; + + + , good color; + + + + , deep color. Since in some cases only 10 or 20 drops of latex were available, other, more accurate, tests could not be used. Only the reactions with triolein and sperm oil are tabulated, but results with other oils will be descri
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