. The development of a cosmid map of chromosome 12p13. Sweet Briar College; Chromosomes. III. Results CHAIN REACTION. PCR reactions were performed on two individual cosmids, cl70G6 and cl28D6. and a set of cosniid pools during this project. Results were successful in that the two individual cosmids were mapped to STS markers on chromosome 12 and cl28D6 was used to determine the location of one particular STS within the region, D12S1914. PCR was also used to label STS markers in the region with digoxigenin for testing with cosmids in hybridization procedures. Prelimi
. The development of a cosmid map of chromosome 12p13. Sweet Briar College; Chromosomes. III. Results CHAIN REACTION. PCR reactions were performed on two individual cosmids, cl70G6 and cl28D6. and a set of cosniid pools during this project. Results were successful in that the two individual cosmids were mapped to STS markers on chromosome 12 and cl28D6 was used to determine the location of one particular STS within the region, D12S1914. PCR was also used to label STS markers in the region with digoxigenin for testing with cosmids in hybridization procedures. Preliminary Experiments. The preliminary PCR reactions were used to verify the protocols being used and to test the accuracy of the techniques. Using cosmid 170G6 and primers 950F and 950R, ten repetitions of the replication cycle (94°C. 58°C, and 72°C for thirty seconds each) were attempted with no resulting positive band when electrophoresed. A repeat of the experiment using thirty repetitions of the replication cycle gave a positive band for both the cosmid and the total human DNA columns (FIGURE 16).. FiouRE 16. PCR of Cosmid I70G6 with Forward and Reverse 950 Primers. Positive band for D12S950. Lane 1 is empty. Lane 2 contains 1 KB ladder. Lane 3 contains Total Human DNA (K562), Lane 4 contains Chromosome 12, and Lane ."i contains cosmid 170G6. There was no band for chromosome 12 as was expected following observation. The signal fi-om chromosome 12 DNA, obtained from a rodent-human cell hybrid, should have shown 32. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Belk, Stephanie Heather.
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Keywords: ., bookcentury1900, booksubjectchromo, booksubjectsweetbriarcollege
